ACETONE-BUTANOL-ETHANOL FERMENTATION FOR BIOENERGY USING VARIOUS SUBSTRATES IN DEFINED TYA MEDIA

Hanies Ambarsari, Kenji Sonomoto

Abstract


A series of experiments had been conducted to investigate the performance of Clostridium
saccharoperbutylacetonicum strain N1-4 (ATCC 13564) in direct fermentation of acetone, butanol, and
ethanol (ABE) using several substrates that have not been investigated previously using this strain,
such as cellobiose, dextrin, starches of corn, potato, tapioca, wheat, and sago, as well as more
complex carbohydrates such as xylans, cellulose, and carboxyl methyl cellulose (CMC), particularly to
study the effect of such various substrate types and concentrations, as well as the substrates mixtures
on the ABE ratio and productivity. The results showed that strain N1-4 could directly produce ABE from
various substrates, from monosaccharide to polysaccharides of hexose or pentose sugars, except
CMC and cellulose. The experiments also suggested that cellobiose (a hexose disaccharide) could be
the more efficient substrate for strain N1-4 than glucose (a monosaccharide) to produce ABE in the TYA
(Tryptone Yeast Agar) medium. It was proven also that the ABE ratio was very dependent on the type
and concentration of substrates being used by strain N1-4.
Keywords: acetone, butanol, ethanol, clostridium saccharoperbutylacetonicum N1-4, fermentation


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DOI: http://dx.doi.org/10.29122/elk.v11i1.1590

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