PERAN MUTASI GEN ACY II TERHADAP PRODUKSI ANTIBIOTIK SEFALOSPORIN

Indria Puti Mustika, Ahmad Wibisana

Abstract


The Roles of AcyII Gene Mutations for Production of AntibioticDerived From Cephalosporin

Semisynthetic antibiotics cephalosporins are widely used to treat infectious diseases, especially those caused by gram-negative bacteria. Various types of semisynthetic antibiotics could be synthesized using 7-aminocephalosporanic acid (7-ACA) as the main raw material. 7-ACA is obtained by conversion of cephalosporin C, either chemically or enzymatically. Converting cephalosporin C to 7-ACA enzymatically in one step involves the cephalosporin acylase enzyme. Currently, all of cefalosporin acylase enzymes produced by wild-type microbes have only high activity on glutaryl-7-ACA as the main substrate. Genetic engineering of the encoding gene of cefalosporin acylase is required to obtain recombinant enzyme having high activity on cephalosporin C. In this paper, the engineering attempts made on acyII gene from Pseudomonas SE83 using directed mutagenesis, error prone PCR, and structural modeling are described. 

Keywords: AcyII gene, cephalosporin, cephalosporin C acylase, enzyme activity, mutation

 

ABSTRAK

Antibiotik sefalosporin semisintetik banyak digunakan untuk mengatasi penyakit infeksi, khususnya yang ditimbulkan oleh bakteri gram negatif. Berbagai jenis antibiotik semisintetk dapat disintesis menggunakan senyawa asam 7-aminosefalosporanat (7-ACA) sebagai bahan baku utamanya. Senyawa 7-ACA diperoleh melalui konversi sefalosporin C, baik yang dilakukan secara kimiawi maupun enzimatis. Konversi sefalosporin C menjadi 7-ACA secara enzimatis dalam satu langkah melibatkan enzim sefalosporin asilase. Hingga saat ini, seluruh enzim sefalosporin asilase yang dihasilkan oleh mikroba wild type hanya mempunyai aktifitas yang tinggi terhadap glutaryl-7-ACA. Rekayasa genetik terhadap gen pengkode enzim sefalosporin asilase diperlukan untuk memperoleh enzim rekombinan yang mempunyai aktifitas tinggi terhadap substrat sefalosporin C. Dalam ulasan ini diuraikan upaya-upaya rekayasa yang telah dilakukan terhadap gen acyII dari Pseudomonas SE83 menggunakan teknik mutasi terarah, error prone PCR, dan pemodelan struktur.

Kata kunci: Aktivitas enzim, gen acyII, mutasi, sefalosporin, sefalosporin C asilase

 

Received: 14September 2017    Accepted: 19 December 2017     Published: 30 December 2017  

  

Received: 14September 2017                Accepted: 19 December 2017           Published: 30 December 2017 

 

Keywords


AcyII gene; cephalosporin; cephalosporin C acylase; enzyme activity; mutation

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References


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DOI: http://dx.doi.org/10.29122/jbbi.v4i2.2272

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