Intan Permatasari, Rully Adi Nugroho, Vincentia Irene Meitiniarti


Decolorization of Sumifix Blue and Reactive Red 2 Textile Dyes by Microbes Isolated from Textile Waste Water

Azo dyes represent the most commonly used group of dyes in textile industry and discharged into industrial effluents worldwide. Aims of this study are to isolate microbe from textile waste water and to determine their ability to decolorize Sumifix Blue and Reactive Red 2 textile dyes. Microbe was isolated from textile effluent of PT Timatex, Salatiga. The activity for decolorization was assayed by inoculating microbial isolates into dye containing medium. Living and nonliving cell were incubated in dye containing medium in order to determine if microbial cells involved in decolorizing dye. Five different microbial isolates have been isolated from textile waste water.  Isolates IBLTT_1 and IBLTT_5 showed the highest activity to decolorize Sumifix Blue, and only isolate IBLTT_1 showed the highest capability in decolorizing Reactive Red 2. Both isolates indicated positive potential towards biotreatment of textile waste water. Further results confirmed that decolorization was due to biodegradation, rather than physical adsorption by inactive cells.

Keywords: decolorization, microbial isolation, Reactive Red 2, Sumifix Blue, textile effluent



Pewarna azo mewakili kelompok pewarna yang umum digunakan pada industri tekstil dan banyak dijumpai di buangan limbah industri tekstil. Tujuan dari penelitian ini adalah untuk mendapatkan isolat dari limbah tekstil dan untuk mengetahui kemampuannya dalam mendekolorisasi pewarna tekstil Sumifix Blue dan Reactive Red 2. Sampel diperoleh dari limbah industri tekstil PT Timatex, Salatiga. Uji kemampuan dekolorisasi dilakukan dengan menginokulasikan isolat mikroba ke dalam medium Nutrient Broth yang mengandung pewarna. Untuk mengetahui apakah sel mikroba terlibat dalam dekolorisasi pewarna, maka sel hidup dan mati diinokulasi pada medium tersebut. Lima isolat yang berbeda diperoleh dalam penelitian ini. Isolat IBLTT_1 dan IBLTT_5 merupakan isolat dengan kemampuan dekolorisasi Sumifix Blue tertinggi. Isolat IBLTT_1 juga merupakan isolat dengan kemampuan dekolorisasi Reactive Red 2 tertinggi. Kedua isolat tersebut menunjukkan potensi positif terhadap pengolahan limbah tekstil. Hasil lebih lanjut menegaskan bahwa dekolorisasi Sumifix Blue dan Reactive Red 2 disebabkan oleh proses biodegradasi, bukan diadsorpsi oleh sel yang mati.

Kata kunci: dekolorisasi, isolat mikroba, limbah tekstil, Reactive Red 2, Sumifix Blue


decolorization; microbial isolation; Reactive Red 2; Sumifix Blue; textile effluent

Full Text:



Abo-State MAM, Saleh YE, Hazaa HA (2017) Decolorization of congo red dye by bacterial isolates. J Eco Heal Env 5:41-48. Doi: 10.12785/jehe/050201

Abubacker MN, Mehala T (2014) Decolourization of directorange-102 and malachite green by bacterial consortium. BiolifeJ 2:1293-1300

Al-Garni SM, Ghanem KM, Kabli SA, Biag AK (2013) Decolorization of crystal violet by mono and mixed bacterial culture techniques using optimized culture conditions. Pol J Environ Stud 22: 1297-1306

Asad S, Amoozegar MA, Pourbabaee AA, Sarbolouki MN, Dastgheib SMM (2007) Decolorization of textile azo dyes by newly isolated halophilic and halotolerant bacteria. Bioresour Technol 98:2082-2088. Doi:10.1016/j.biortech.2006.08.020

Athira S, Gowdhaman D, Ponnusami V (2016) Molecular characterization and azoreductase activity of Pseudomonas stutzeri isolated from textile dye effluent. Int J Chem Sci 14: 2703-2709

Brüschweiler BJ, Küng S, Bürgi D, Muralt L, Nyfeler E (2014) Identification of non-regulated aromatic amines of toxicological concern which can be cleaved from azo dyes used in clothing textiles. Regul Toxicol Pharmacol 69: 263-272. Doi: 10.1016/j.yrtph.2014.04.011

Chacko JT, Subramaniam K (2011) Enzymatic degradation of azo dyes - Areview. Int J Environ Sci 1: 1250-1260

Emilia TA, Wijaya YA, Mermaliandi F (2016) Degradation of reactive red 2 by fenton and photo-fenton oxidation processes. J Eng Appl Sci 11:5227-5231.

Franciscon E, Grossman MJ, Paschoal JA, Reyes FG, Durrant LR (2012) Decolorization and biodegradation of reactive sulfonate azo dyes by a newly isolated Brevibacterium sp. strain VN-15. Springerplus 1: 37-46. Doi: 10.1186/2193-1801-1-37

Handayani W, Meitiniarti VI, Timotius KH (2007) Decolorization of acid red 27 and reactive red 2 by Enterococcus faecalis under a batch system. World J Microbiol Biotechnol 23: 1239-1244. Doi: 10.1007/s11274-007-9355-1

Hassan MM, Alam MZ, Anwar MN (2013) Biodegradation of textile azo dyes by bacteria isolated from dyeing industry effluent. Int Res J Biological Sci 2: 27-31

Jaiswal SS, Gomashe AV (2017) Bioremediation of textile azo dyes by newly isolated Bacillus sp. from dye contaminated soil. Int J Biotechnol Biochem 13:147-153

Karthikeyan G, Radha T, Ragunathan R, Johnney J (2016) Degradation and decolourisation of dye effluents by using Bacillus subtilis CBNR isolates. Int Res J Multidisciplinary Sci Technol 1: 142-147

Khehra MS, Saini HS, Sharma DK, Chadha BS, Chimni SS (2005) Comparative studies on potential of consortium and constituent pure bacterial isolates to decolorize azo dyes. Water Res 39: 5135-5141. Doi: 10.1016/j.watres.2005.09.033.   

Khalid A, Arshad M, Crowley DE (2008) Decolorization of azo dyes by Shewanella sp. under saline conditions. Appl Microbiol Biotechnol 79: 1053-1059. Doi: 10.1007/s00253-008-1498-y

Kiernan JA (2001) Classification and naming of dyes, stains and fluorochromes. Biotech Histochem 76: 261-278. Doi: 10.1080/bih.76.5-6.261.278

Leelakriangsak M, Borisut S (2012) Characterization of the decolorizing activity of azo dyes by Bacillus subtilis azoreductase AzoR1. Songklanakarin J Sci Technol 34: 509-516

Lalnunhlimi S, Krishnaswamy V (2016) Decolorization of azo dyes (Direct Blue 151 and Direct Red 31) by moderately alkaliphilic bacterial consortium. Braz J Microbiol 47: 39-46. Doi: 10.1016/j.bjm.2015.11.013

Maheswari NU, Sivagami S (2016) Biological degradation of textile dyes using marine Bacillus species. Int J Pure App Biosci 4: 123-128. Doi: 10.18782/2320-7051.2326

Mumtaz S, Malik NH, Naz I, Ahmed S (2015) Decolorization of textile dye reactive blue 221 by bacteria isolated from anthropogenic dye-contaminated soil. Pol J Environ Stud 24: 1705-1716

Naresh B, Preethi C, Sneha S, Bhagyashree R, Parizad P (2013) Microbial decolorization of disperse textile dye brown 21 by Enterobacter gergoviae isolated from textile effluent. Int Res J Environ Sci 2:31-36

Rawat D, Mishra V, Sharma RS (2016) Detoxification of azo dyes in the context of environmental processes. Chemosphere 155: 591-605.  Doi: 10.1016/j.chemosphere.2016.04.068

Shah MP (2014) Biodegradation of azo dyes by three isolated bacterial strains: An environmental bioremedial approach. J Microb Biochem Technol S3:007. Doi: 10.4172/1948-5948.S3-007

Shah MP, Patel KA, Nair SS, Darji AM (2013) Microbial degradation of textile dye (Remazol Black B) by Bacillus spp. ETL-2012. J Bioremed Biodeg 4: 180-184.Doi: 10.4172/2155-6199.1000180

Sharma S, Munjal A, Gupta S (2011) Comparative studies on decolorization of textile azo dyes by different bacterial consortia and pure bacterial isolate. J Pharm Res 4: 3180-3183

Sriram N dan Reetha D (2015) Isolation and characterization of dye degrading bacteria from textile dye effluents.  Cent Euro J Exp Biol 4: 5-10

Van der Zee FP, Lettinga G, Field JA (2001) Azo dye decolorisation by anaerobic granular sludge. Chemosphere 44:1169-1176

Velmurugan S, Revikumar R (2014) Biodegradation and decolorization of reacive dye red ME4BL by Bacillus subtilis. Int J of Environ Bioremed Biodeg 2: 250-255

Vimala G, Jeyakumar P, Devi AC, Singh A, Iyer P (2015) Azo dye degrading bacteria from textile effluent. Int J Curr Microbiol App Sci 4: 199-210

Yan MP, Razak A, Bakar Wan AB (2003) Photocatalytic degradation of sumifix blue. Borneo Sci 13: 63-69



  • There are currently no refbacks.

© Copyright: BY-NC-SA