AKTIVITAS Stenotrophomonas rhizophila DAN Trichoderma sp. DALAM MENGHAMBAT PERTUMBUHAN Ganoderma boninense

Bedah Rupaedah, Debby Viola Amanda, Reni Indrayanti, Nia Asiani, Bambang Sukmadi, Asep Ali, Abdul Wahid, Taufik Firmansyah, Mahmud Sugianto

Abstract


Activities of Stenotrophomonas rhizophila and Trichoderma sp. in Inhibiting the Growth of Ganoderma boninense 

ABSTRACT

Basal stem rot (BSR) disease in oil palm (Elaeis guineensis Jacq.) due to infection of Ganoderma boninense. Various efforts to overcome BSR disease has been done, such as by utilizing endophytic microbes. The purpose of this research was to determine the activities of Stenotrophomonas rhizophila and Trichoderma sp inhibiting the growth of G. boninense. This research was divided into three stages, namely: stability test of S. rhizophila activity against G. boninense; activity of chitinase and cellulase enzymes produced by S. rhizophila; the effectiveness of S. rhizophila and Trichoderma sp. on G. boninense in a greenhouse. The parameters observed were plant height, leaves number, chlorophyll content, disease incidence and severity. The stability testing of S. rhizophila activity against G. boninense showed 53% of inhibition. Chitinase activity showed negative result. While cellulase index was about 0.46. The effectiveness test showed the significantly different results on plant height, leaves number and chlorophyll content.

Keywords: Chitinase, cellulase, Ganoderma boninense, Stenotrophomonas rhizophila, Trichoderma sp.

 

ABSTRAK

Penyakit busuk pangkal batang (BPB) pada tanaman kelapa sawit (Elaeis guineensis Jacq.) muncul karena diinfeksi oleh Ganoderma boninense. Berbagai upaya penanggulangan penyakit BPB telah dilakukan, diantaranya dengan memanfaatkan mikroba endofit. Tujuan dari penelitian ini adalah untuk mengetahui aktivitas bakteri Stenotrophomonas rhizophila dan Trichoderma sp. dalam menghambat pertumbuhan G. boninense. Penelitian ini dibagi menjadi tiga tahapan, yaitu: pengujian stabilitas aktivitas S. rhizophila terhadap G. boninense; pengujian aktivitas enzim kitinase dan selulase yang dihasilkan oleh S. rhizophila; pengujian efektivitas S. rhizophila dan Trichoderma sp. terhadap G. boninense di rumah kaca. Parameter yang diamati pada pengujian efektivitas berupa tinggi tanaman, jumlah daun, jumlah klorofil, kejadian dan keparahan penyakit. Uji stabilitas aktivitas S. rhizophila terhadap G. boninense menunjukkan adanya penghambatan rata-rata sebesar 53%. Uji aktivitas enzim kitinase pada bakteri S. rhizophila menunjukkan hasil negatif. Sedangkan indeks enzim selulase pada bakteri S. rhizophila sebesar 0.46. Pada uji efektivitas tampak hasil yang berbeda nyata pada parameter tinggi tanaman, jumlah daun dan kandungan klorofil.

Kata Kunci: Kitinase, selulase, Ganoderma boninense, Stenotrophomonas rhizophila,    Trichoderma sp.


Keywords


Chitinase; cellulase; Ganoderma boninense; Stenotrophomonas rhizophila; Trichoderma sp

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DOI: http://dx.doi.org/10.29122/jbbi.v5i1.2767

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