VARIASI GENETIK KAMBING BENGGALA DI KABUPATEN MANGGARAI BARAT BERDASARKAN METODE RANDOM AMPLIFIED POLYMORPHIC DNA

Suhendra Pakpahan, Wayan Tunas Artama, Rini Widayanti, I Gede Suparta Budisatria

Abstract


Genetic Variation of Benggala Goats in West Manggarai Regency Based on Random Amplified Polymorphic DNA Method

 

ABSTRACT

Indonesia has several types of local goats that have had an extended period of adaptation to the natural conditions in Indonesia. Goat is one of the most important germplasm in supporting the economy of rural communities. Benggala is a local breed of goat originating from Flores Island, East Nusa Tenggara province and has distinctive characteristics. The RAPD technique has several advantages and has been widely used in studies of the genetic diversity of goats. A total of 50 blood samples of Benggala goats were taken from four sub-districts in West Manggarai Regency. This study was conducted to estimate genetic variations of Benggala goats using OPA-6 and OPA-16 primers. The OPA-6 primer consisted of 0-11 bands, while the OPA-16 primer consisted of 0-7 bands. The total bands produced on the OPA-6 primer from all samples was 456, whilst OPA-16 primer was 314. The lowest genetic similarity between individuals of Benggala goats was 44% from the sample K46. Based on the sample population, the average genetic similarity level was 72%. These results show that the genetic diversity of Benggala goats is low.

Keywords: Benggala  goat, genetic similarity,genetic variation, RAPD, West Manggarai

 

ABSTRAK

Indonesia memiliki beberapa jenis kambing lokal yang memiliki periode adaptasi yang panjang dengan kondisi alam di Indonesia. Kambing merupakan salah satu plasma nutfah yang sangat penting dalam mendukung perekonomian masyarakat pedesaan. Benggala adalah jenis kambing lokal yang berasal dari pulau Flores, propinsi Nusa Tenggara Timur dan kambing Benggala memiliki ciri khas. Teknik RAPD memiliki beberapa keunggulan dan telah banyak digunakan pada studi keragaman genetik kambing. Total 50 sampel darah kambing Benggala yang diambil dari empat kecamatan di Kabupaten Manggarai Barat. Penelitian ini dilakukan untuk menguji variasi genetik kambing Benggala dengan menggunakan primer OPA-6 dan OPA-16. Primer OPA-6 terdiri dari 0-11 band, sedangkan primer OPA-16 terdiri dari 0-7 band. Total jumlah pita yang dihasilkan pada primer OPA-6 dari semua sampel adalah 456, sementara primer OPA-16 adalah 314. Kemiripan genetik terendah antara individu-individu kambing Benggala adalah 44% dari sampel K46. Berdasarkan populasi sampel, tingkat kemiripan genetik rata-rata adalah 72%. Hasil ini menunjukkan bahwa keragaman genetik kambing Benggala tergolong rendah.

Kata Kunci: kambing Benggala, kemiripan genetik, Manggarai Barat , RAPD, variasi genetik


Keywords


Genetic variation; Benggala goat; RAPD; genetic similarity; Indonesia

Full Text:

PDF

References


Al-Barzinji YMS, Oramari RA, Al-Sanjury RA (2016) Molecular characterization of Iraqi local goat breeds using random amplified polymorphic DNA Markers. Iranian J Appl Anim Sci 6:671-678

Aziz MA (2010) Present status of the world goat populations and their productivity. Lohmann Information 45:42-52

Batubara A (2011) Studi keragaman fenotipik dan genetik beberapa sub populasi kambing lokal Indonesia dan strategi pemanfaatannya secara berkelanjutan. Tesis, Institut Pertanian Bogor

Batubara A, Noor RR, Farajallah A, Tiesnamurti B (2013) Keragaman genetik DNA Y-kromosom pada enam rumpun kambing lokal Indonesia. Pp 316-325. Prosiding Seminar Nasional Teknologi Peternakan dan Veteriner, September 3-5, 2013. lAARD Press, Jakarta

El-tarras AE, Shahaby AF, Banaja AE (2015) Assessment of genetic diversity in Saudi goats, Saudi Arabia using genetic finger printing. Int J Curr Microbiol App Sci 4:223-231

Al-Otaibi SA, Fahmi AL (2011) Genetic variation in captive herd of Arabian Oryx using RAPD and ISSR markers. Afr J Biotechnol 10:5251-5262. doi: 10.5897/AJB10.1162

Kumar D, Dixit SP, Sharma R, Pandey AK, Sirohi G, Patel AK, Aggarwal RAK, Verma NK, Gour DS, Ahlawat SPS (2005) Population structure, genetic variation and management of Marwari goats. Small Ruminant Res 59:41-48. doi: 0.1016/j.smallrumres.2004.11.013

Kumar NS, Gurusubramanian G (2011) Random amplified polymorphic DNA (RAPD) markers and its applications. Sci Vis 11:116-124

Kumar S, Kolte AP, Yadav BR, Kumar S, Arora AL, Singh VK (2008) Genetic variability among sheep breeds by random amplified polymorphic DNA-PCR. Indian J Biotechnol 7:482-486

Kumari N, Thakur SK (2014) Randomly amplified polymorphic DNA: A brief review. Am J Anim Vet Sci 9:6-13. doi: 10.3844/ajavsp.2014.6.13

Kumari N, Singh LB, and Kumar S (2013)    Molecular characterization of goats using random amplified polymorphic DNA. Am J Anim Vet Sci 8:45-49. doi: 10.3844/ajavsp.2013.45.49

Mudawamah M, Retnaningtyas ID, Wadjdi MF, Badriyah B, Susilowati S, Aulanni’am A, Ciptadi G (2014) Analisis kemiripan genetika antara kambing peranakan ettawa hasil kawin alam dengan inseminasi buatan berdasarkan RAPD. J Kedokteran Hewan 8:137-140. doi: 10.21157/j.ked.hewan.v8i2.2636

Nezhad NM, Solouki M, Siasar B (2010) The comparison of long and short primers used for RAPD technique in grape. Trakia J Sci 8:38-41

Pakpahan S, Artama WT, Widayanti R, Suparta IG (2015) Genetic variations and the origin of native Indonesian goat breeds baseds on mtDNA D-Loop sequences. Asian J Anim Sci 9:341-350. doi: 10.3923/ajas.2015.341.350

Pakpahan S, Artama WT, Widayanti R, Suparta IG (2016) Genetic characteristics and relationship in different goat populations of indonesia based on cytochrome B gene sequences. Asian J Anim Sci 10:29-38 doi: 10.3923/ajas.2016.29.38

Pamungkas FA, Batubara A, Doloksaribu M, Sihite E (2009) Petunjuk Teknis: Potensi plasma nutfah kambing lokal Indonesia. Puslitbang Peternakan, Badan Litbang Pertanian, Departemen Pertanian

Rao KA, Bhat KV, Totey SM (1996) Detection of species-specific genetic markers in farm animals through random amplified polymorphic DNA (RAPD). Genet Anal: Biomol Eng 13:135-138

Rashidi A, Mokhtari MS, Gutiérrez JP (2015) Pedigree analysis and inbreeding effects on early growth traits and greasy fleece weight in Markhoz goat. Small Ruminant Res 124:1-8. doi: 10.1016/j.smallrumres.2014.12.011

Rohlf FJ (2000) NTSYS-pc: Numerical Taxonomy and Multivariate Analysis System Version 2.1. User Guide. Department of Ecology and Evolution State University of New York. ISBN: 0-925031-30-5

Rout PK, Joshi MB, Mandal A, Laloë D, Singh L, Thangaraj K (2008) Microsatellite-based phylogeny of Indian domestic goats. BMC genetics 9:11. doi: 10.1186/1471-2156-9-11

Ruane P, Lang J, DeJesus E, Berger DS, Dretler R, Rodriguez A, Shaefer MS (2006) Pilot study of once-daily simplification therapy with abacavir/lamivudine/zidovudine and efavirenz for treatment of HIV-1 infection. HIV Clinical Trials 7:229-236. doi: 10.1310/hct0705-229

Sabir JS, Mutawakil MH, El-Hanafy AA, Ahmed MM (2012) Genetics similarity among four breeds of goat in Saudi Arabia detected by random amplified polymorphic DNA marker. Afr J Biotechnol 11:3958-3963. doi: 10.5897/AJB11.4120

Sulaiman BK (2012) Evaluation of the genetic diversity in Iraqi local breeds of goat using random amplified polymorphic DNA markers. Al-Anbar J Vet Sci 5:179-186




DOI: http://dx.doi.org/10.29122/jbbi.v5i2.2943

Refbacks

  • There are currently no refbacks.


© Copyright: BY-NC-SA