IDENTIFIKASI AKTINOMISETES SEDIMEN AIR TAWAR MAMASA, SULAWESI BARAT DAN AKTIVITASNYA SEBAGAI ANTIBAKTERI DAN PELARUT FOSFAT

Ade Lia Putri, Puspita Lisdiyanti, Mia Kusmiati

Abstract


Identification of Actinomycetes in Freshwater Sediments from Mamasa, West Sulawesi and Their Antibacterial and Phosphate Solubilizing Activities


ABSTRACT

A large number of actinomycetes that have been isolated and screened were obtained from soil and marine samples. Consequently, the possibility of isolating novel Actinomycetes and secondary metabolites compounds strains from soil and marine samples have limited. Exploration of actinomycetes from freshwater sediment is rare. In this study, 30 isolates of Actinomycetes from freshwater sediments in Mamasa District, West Sulawesi were isolated, identified, and screened for their antibacterial and phosphate solubilizing activity. Actinomycetes were isolated by serial dilution method and were identified based on morphological and 16S rRNA gene sequence. Antibiotic activity was screened using the agar plug diffusion method, while soluble phosphate ability was observed by clear zone ratio in PKA medium. Most of the isolates belong to the genus Streptomyces (80%). Out of 30 isolates, 56.6% showed antibacterial activity and 36.6% had potential as solubilizing phosphate which belong to genus Streptomyces, Actinomadura, and Kitasatospora.

Keywords: 16S rRNA, Actinomycetes, antibacterial, freshwater sediment, phosphate solubilizing

 

ABSTRAK

Sebagian besar aktinomisetes yang telah diisolasi dan dilakukan penapisan metabolit sekundernya berasal dari sampel tanah dan laut. Konsekuensinya, kesempatan untuk menemukan aktinomisetes jenis baru maupun yang menghasilkan metabolit sekunder baru dari tanah dan laut semakin berkurang. Eksplorasi aktinomisetes dari lingkungan lain seperti sedimen air tawar jarang dilakukan. Pada penelitian ini, 30 isolat aktinomisetes yang diisolasi dari sedimen air tawar di Kabupaten Mamasa, Sulawesi Barat, telah diidentifikasi dan dilakukan penapisan antibakteri dan kemampuan isolat dalam melarutkan fosfat. Aktinomisetes diisolasi dengan metode pengenceran secara langsung dan selanjutnya diidentifikasi secara morfologi dan molekular berdasarkan gen 16S rRNA. Metode yang digunakan dalam penapisan aktivitas antibakteri adalah agar plug diffusion method, sedangkan kemampuan aktinomisetes dalam melarutkan fosfat diuji dengan cara menumbuhkan isolat pada media PKA. Isolat yang paling banyak diisolasi termasuk ke dalam marga Streptomyces (80%). Dari 30 isolat, 56,6% isolat menunjukkan adanya aktivitas antibakteri dan 36,6% dari isolat berpotensi sebagai pelarut fosfat, yang termasuk ke dalam marga Streptomyces, Actinomadura, dan Kitasatospora.

Kata Kunci: 16S rRNA, Aktinomisetes, sedimen air tawar, antibakteri, pelarut fosfat


Keywords


16S rRNA; Aktinomisetes; sedimen air tawar; antibakteri; pelarut fosfat

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DOI: http://dx.doi.org/10.29122/jbbi.v5i2.2953

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