PENGARUH WADAH KULTUR DAN KONSENTRASI SUMBER KARBON PADA PERBANYAKAN KENTANG ATLANTIK SECARA IN VITRO

Karyanti Karyanti, Yosua Glen Kristianto, Hayat Khairiyah, Linda Novita, Tati Sukarnih, Yayan Rudiyana, Dewi Yustika Sofia

Abstract


The Effect of Culture Container and Carbon Source Concentration on In Vitro Shoot Propagation of Atlantic Potato


ABSTRACT

Potato is a food commodity that has the potential to support food diversification in Indonesia. There is an increasing demand for Atlantic potatoes as the raw material for processed potato products. The demand, which has not been met by the increased production, has been the cause of the ongoing potato import activities in Indonesia. The limitation of producing quality Atlantic potato seeds economically is one of the obstacles to increasing the production of Atlantic potatoes in Indonesia. The aim of this research was to study the effect of various table sugar concentrations as the carbon source and the type of the culture containers used for Atlantic potato shoot multiplication in vitro. The propagation was carried out in bioreactors and culture bottles with MS liquid medium + coconut water at a concentration of 150 mL/L medium, and 3 concentration levels of table sugar, namely 0; 7.5; and 15 g/L medium. The use of bioreactor significantly increased the height of the Atlantic potato plantlets. The use of bioreactor combined with table sugar addition decreased hyperhydricity level. The highest number of shoots, leaves, and roots were found at the table sugar concentration of 15 g/L medium in both containers.

Keywords: bioreactor, micropropagation, shoot culture, Solanum tuberosum, sucrose

 

ABSTRAK

Kentang merupakan komoditas pangan yang berpotensi mendukung program diversifikasi pangan di Indonesia. Peningkatan permintaan terhadap kentang Atlantik sebagai bahan baku kentang olahan yang tak diimbangi dengan peningkatan produksi kentang Atlantik menjadi penyebab masih berlangsungnya impor kentang Atlantik di Indonesia. Keterbatasan menghasilkan benih kentang Atlantik berkualitas yang ekonomis merupakan salah satu hambatan dalam meningkatkan produksi kentang Atlantik di Indonesia. Penelitian ini bertujuan mempelajari pengaruh variasi konsentrasi sukrosa teknis sebagai sumber karbon dan penggunaan jenis wadah terhadap perbanyakkan tunas kentang Atlantik secara in vitro. Perbanyakkan tunas kentang Atlantik menggunakan media MS cair + 150 mL/L air kelapa dalam wadah bioreaktor dan botol kultur dengan 3 taraf konsentrasi sukrosa, yaitu 0; 7,5; dan 15 g/L media. Penggunaan bioreaktor secara signifikan meningkatkan tinggi planlet kentang Atlantik yang dihasilkan. Penggunaan bioreaktor yang dikombinasikan dengan penambahan sukrosa teknis menurunkan tingkat hiperhidrisitas. Tunas, daun, dan akar terbanyak dihasilkan oleh perlakuan sukrosa teknis 15 g/L media dalam kedua jenis wadah.

Kata Kunci: bioreaktor, kultur tunas, mikropropagasi, Solanum tuberosum, sukrosa


Keywords


bioreactor; micropropagation; shoot culture; Solanum tuberosum; sucrose

Full Text:

PDF

References


Afreen F (2008) Temporary Immersion Bioreactor: Engineering considerations and applications in plant micropropagation. In: Gupta SD and Ibaraki Y (ed) Plant Tissue Culture Engineering. Springer, Dordrecht, pp 187-201

Asgar A, Rahayu ST, Kusmana, Sofiari E (2011) Uji kualitas umbi beberapa klon kentang untuk keripik. J Hort 21:51-59. doi: 10.21082/jhort.v21n1.2011.p51-59

BI (2011) Pola Pembiayaan Usaha Kecil: Budidaya Kentang Industri. Direktorat Kredit, BPR dan UMKM. Bank Indonesia, Jakarta

BPTP (2014) Mengenal beberapa varietas kentang dan manfaatnya. Lembar Informasi Pertanian No. 04/DH/2014. Badan Pengkajian Teknologi Pertanian, Sumatera Selatan

Cassells AC (2016) Tissue Culture: Micropropagation In: Thomas B, Murphy DJ, Murray BG (ed) Encyclopedia of Applied Plant Sciences. Second Edition. Elsevier, London

Dinarti Diny (2012) Perbanyakan dan induksi umbi lapis mikro bawang merah secara in vitro. Disertasi, Institut Pertanian Bogor

Ebadi M, Iranbakhsh A, Khaniki BG (2007) Shoot micropropagation and microtuberization in potato (Solanum tuberosum L.) by the semi-continuous bioreactor. Pak J Biol Sci 10:861-867. doi: 10.3923/pjbs.2007.861.867

FAO (2008) Potatoes, nutrition and diet. International Year of the Potato Secretariat, Food and Agriculture Organization, Rome

Handayani T dan Karjadi AK (2014) Varietas Unggul Baru (VUB) kentang menjawab kebutuhan bahan baku olahan. Balai Penelitian Tanaman Sayur, Bandung

Ibrahim MSD, Hartati RS, Rubiyo, Purwito A, Sudarsono (2017) Efisiensi media kultur dan aplikasi temporary immersion system pada embriogenesis somatik kopi arabika. J Littri 23:45-54. doi: 10.21082/littri.v23n1.2017.45-54

Ikeuchi M, Sugimoto K, Iwase A (2013) Plant callus: Mechanisms of induction and repression. Plant Cell 25:3159-3173. doi: 10.1105/tpc.113.116053

Indriani BS (2014) Efektivitas subtitusi sitokinin dengan air kelapa pada medium multiplikasi tunas krisan (Chrysanthemum indicum L.) secara in vitro. Skripsi, Universitas Negeri Semarang

Jeong JA, Wu CH, Murthy HN, Hahn EJ, Paek KY (2009) Application of an airlift bioreactor system for the production of adventitious root biomass and caffeic acid derivatives of Echinacea purpurea. Biotechnol Bioproc E 14:91-98. doi: 10.1007/s12257-007-0142-5

Kristina NN dan Syahid SF (2014) Air kelapa sebagai hormon tumbuh dalam kultur in vitro temu lawak. Warta Penelitian dan Pengembangan Tanaman Industri 20:7-9

Kusandriani Y (2014) Uji daya hasil dan kualitas delapan genotip kentang untuk industri keripik kentang nasional berbahan baku lokal. J Hort 24:283-288

Kusmana K (2012) Uji adaptasi klon kentang hasil persilangan varietas atlantik sebagai bahan baku keripik kentang di dataran tinggi Pengalengan. J Hort 22:342-348 

Liljana KG, Mitrev S, Fidanka T, Mite I (2012) Micropropagation of potato Solanum tuberosum L. Electron J Biol 8:45-49.

Mbiyu M, Muthoni J, Kabira J, Muchira C, Pwaipwai P, Ngaruiya J, Onditi J, Otieno S (2012) Comparing liquid and solid media on the growth of plantlets from three Kenyan potato cultivars. Am J Exp Agric 2:81-89. doi: 10.9734/AJEA/2012/715

Mello MO, Dias CTS, Amaral AFC, Melo M (2001) Growth of Bauhinia forficata Link, Curcuma zedoaria Roscoe and Phaseolus vulgaris L. cell suspension cultures with carbon sources. Sci Agric 58:481-485. doi: 10.1590/S0103-90162001000300007

Metwali EMR and Al-Maghrabi OA (2012) Effectiveness of tissue culture media components on the growth and development of cauliflower (Brassica oleracea var. Botryis) seeding explants in vitro. Afr J Biotechnol 11:14069-14076. doi: 10.5897/AJB12.1984

Muhibuddin, Zakaria AB, Lisan E, Baharuddin (2009) Peningkatan produksi dan mutu benih kentang hasil kultur in-vitro melalui introduksi sistem aeroponik dengan formulasi NPK. Prosiding Seminar Nasional Pekan Kentang 2008. Hal:102-110. Puslitbang Hortikultura, Badan Litbang Pertanian, Kementerian Pertanian, Jakarta

Nurhaimi-Haris, Ayuningtias NS, Suparto IH (2011) Pengaruh ventilasi terhadap morfologi, stomata dan kadar klorofil tunas karet yang diperbanyak melalui microcutting. Menara Perkebunan 79:57-63

Placide R, Clement U, Fracoise U, Vedaste A (2012) Comparative study of effects of table sugar, laboratory grade sucrose and mannitol on growth of banana plantlets under in vitro conditions. Rwanda J 28:76-83. doi: 10.4314/rj.v28i1.6

Pujol AC (2016) Plant cells and suitable bioreactors. Thesis, Universitat de Barcelona.

Saji KV dan Sujatha M (2004) Embryogenesis and plant regeneration in anther culture of sunflower (Helianthus annuus L.). Euphytica 103:1-7. doi: 10.1023/A:1018318625718

Sari L (2005) Optimasi media untuk jumlah daun dan multiplikasi tunas lidah buaya (Aloe vera) dengan pemberian BAP dan Adenin. Biodiversitas 6:178-180. doi: 10.13057/biodiv/d060308

Sigma Aldrich (2018) Product Information Sucrose Grade II Plant Cell Culture. Sigma Aldrich, Saint Louis, Missouri, US. https://www.sigmaaldrich.com/catalog/product/sigma/s5391?lang=en&region=ID. Diakses 1 Juni 2018

Taskin H, Baktemur G, Kurul M, Buyukalaca S (2013) Use of tissue culture techniques for producing virus-free plant in garlic and their identification through Real-Time PCR. TheScientificWorldJournal 2013:1-5. doi: 10.1155/2013/781282

Vinterhalter D, Dragicevic I, Vinterhalter B (2008) Potato in vitro culture techniques and biotechnology. Fruit, Vegetable, and Cereal Science and Biotechnology 2:16-45

Vyas S, Rao MS, Suthar RK, Purohit SD (2008) Liquid culture system stimulates in vitro growth and shoot multiplication in four medicinally important plants. Medicinal and Aromatic Plant Science and Biotechnology 2:96-100

Wahyurini E (2010) Pengaruh sukrosa terhadap pertumbuhan eksplan tanaman kedelai hitam (Glycine soja) secara in vitro. Hal 157-163. Prosiding Seminar Hasil Penelitian Tanaman Aneka Kacang dan Umbi. Denpasar, 29 Juni 2010. Universitas Pendidikan Nasional

Whitehouse AB, Marks TR, Edwards GA (2002) Control of hyperhydricity in Eucalyptus axillary shoot cultures grown in liquid medium. Plant Cell, Tissue Organ Cult 71:245-252. doi: 10.1023/A:1020360120020

Winarto B, Mattjik NA, Purwito A, Marwoto B (2009) Kultur antera anthurium: Pengaruh sukrosa dan glukosa terhadap keberhasilan induksi pembentukan kalus dan regenerasinya. Berk Penel Hayati 14:165-171

Yesil-Celiktas O, Gurel A, Vardar-Sukan F (2010) Large scale cultivation of plant cell and tissue culture in bioreactors. Transworld Research Network 37:1-54

Zakaria D (2010) Pengaruh konsentrasi sukrosa dan BAP (Benzil Amino Purine) dalam media Murashige Skoog (MS) terhadap pertumbuhan dan kandungan reserpine kalus pule pandak (Rauvolfia verticillata Lour). Thesis, Universitas Sebelas Maret

Zavattieri A, Lima M, Sorbal V, Oliveira P, Costa A (2009) Effects carbon source, carbon concentration and culture conditions on in vitro rooting of Pinus pinea L. Microshoots. Acta Hortic 812:173-180. doi: 10.17660/ActaHortic.2009.812.19





DOI: http://dx.doi.org/10.29122/jbbi.v5i2.3012

Refbacks

  • There are currently no refbacks.


© Copyright: BY-NC-SA