AKTIVITAS ANTIOKSIDAN EKSTRAK KAPANG ENDOFIT Cb.Gm.B3 ASAL RANTING KAYU MANIS (Cinnamomum burmanni)

Fauzy Rachman, Nisa Rachmania Mubarik, Partomuan Simanjuntak

Abstract


Antioxidant Activity of Endophytic Fungi Cb.Gm.B3 Extract from Cinnamon (Cinnamomum burmanni) Twigs


ABSTRACT

There are many degenerative diseases that are caused by a free radical effect. Cinnamon (Cinnamomum burmanni) contains cinnamaldehyde compounds that have activity as a powerful antioxidant and fight free radicals. Endophytic fungi can be found in cinnamon plants living symbiotically. Endophytic fungi produce a variety of bioactive metabolites including antioxidants. This research was conducted to isolate endophytic fungi from C. burmanni plant which is active as antioxidant. Endophytic fungi isolation was carried out using surface sterilization method and cultivated in PDA media. Antioxidant activity test was performed using free radical 2.2-diphenyl-1-picrylhydrazyl (DPPH) method. Selected isolates were then identified molecularly to determine their species. A total of nine fungi were isolated from cinnamon twigs. The result showed that the highest antioxidant activity was obtained from Cb.Gm.B3 with IC50 of 13.219 ± 0.755 µg/mL. The selected isolate Cb.Gm.B3 taxonomically has a high similarity with Neofusicoccum parvum isolate PEL23 (Accession no: KY053054.1).

Keywords: antioxidant, Cinnamomum burmanni, 2.2-diphenyl-1-picrylhydrazyl, endophytic fungi, Neofusicoccum parvum

 

ABSTRAK

Kayu manis (Cinnamomum burmanni) mengandung senyawa sinamaldehid yang memiliki aktivitas sebagai antioksidan kuat dan dapat menangkal radikal bebas. Dalam tanaman kayu manis terdapat kapang endofit yang hidup bersimbiosis. Kapang endofit dapat menghasilkan berbagai senyawa metabolit bioaktif termasuk antioksidan. Penelitian ini dilakukan untuk mengisolasi kapang endofit dari tanaman C. burmanni yang aktif sebagai antioksidan. Isolasi kapang endofit dilakukan menggunakan metode sterilisasi permukaan dan ditanam pada media PDA. Pengujian aktivitas antioksidan dilakukan menggunakan metode peredaman radikal bebas dengan reagen 2.2-difenil-1-pikrilhidrazil (DPPH). Isolat terpilih diidentifikasi secara molekuler untuk menentukan spesiesnya. Sebanyak 9 isolat kapang berhasil diisolasi dari jaringan ranting tanaman kayu manis. Aktivitas antioksidan tertinggi (IC50) didapatkan dari isolat Cb.Gm.B3 sebesar 13,219 ± 0,755 µg/mL. Isolat terpilih Cb.Gm.B3 secara taksonomi memiliki tingkat kemiripan yang tinggi dengan Neofusicoccum parvum isolat PEL23 (No. aksesi: KY053054.1).

Kata Kunci: antioksidan, Cinnamomum burmanni, 2.2-difenil-1-pikrilhidrazil, kapang endofit, Neofusicoccum parvum


Keywords


antioksidan; Cinnamomum burmanni; 2.2-difenil-1-pikrilhidrazil; kapang endofit; Neofusicoccum parvum

Full Text:

PDF

References


Akmalasari I, Purwati ES, Dewi RS (2013) Isolasi dan identifikasi jamur endofit tanaman manggis (Garcinia mangostana L.). Biosfera 30:82-89. Doi: 10.20884/1.mib.2013.30.2.131

Banjarnahor  S, Artanti N (2014) Antioxidant properties of flavonoids. Med J Indones 23:239-244. doi: 10.13181/mji.v23i4.1015

Cholisoh Z, Utami W (2008) Aktivitas penangkapan radikal ekstrak etanol 70% biji Jengkol (Archidendron jiringa). Pharmacon 9:33-40

Dai C, Yu B, Li X (2008) Screening of endophytic fungi that promote the growth of Euphorbia pekinensis. Afr J Biotechnol 7:3505-3510

Dudeja SS, Giri R, Saini R, Suneja-Madan P, Kothe E (2012) Interaction of endophytic microbes with legumes. J Basic Microbiol 52:248-260. doi: 10.1002/jobm.201100063

Ferry Y (2013) Prospek pengembangan kayu manis (Cinnamomum burmanii L) di Indonesia.  SIRINOV 1:11-20

Fransworth NR (1966) Biological and phytochemical screening of plant. J Pharm Sci 55:225-265. doi: 10.1002/jps.2600550302

Gagola C, Suryanto E, Wewengkang D (2014) Aktivitas antioksidan dari ekstrak fenolik cortex umbi ubi kayu (Manihot esculenta) daging putih dan daging kuning yang diambil dari kota Melonguane Kabupaten Kepulauan Talaud. Pharmacon J Ilmiah Farmasi 3:127-133

Ghasemzadeh A, Ghasemzadeh N (2011) Flavonoids and phenolic acids: Role and biochemical activity in plants and human. J Med Plants Res 5:6697-6703. doi: 10.5897/JMPR11.1404

Ginting RCB, Sukarno N, Widyastuti U, Darusman LK, Kanaya S (2013) Diversity of endophytic fungi from red ginger (Zingiber officinale Rosc.) plant and their inhibitory effect to Fusarium oxysporum plant pathogenic fungi. Hayati 20:127-137. doi: 10.4308/hjb.20.3.127

Harborne JB (1987) Metode Fitokimia: Penuntun Cara Modern Menganalisa Tumbuhan. Edisi kedua. Terjemahan. Padmawinata K, Soediro I, Niksolihin S. Penerbit ITB, Bandung

Heath RN, Roux J, Slippers B, Drenth A, Pennycook SR, Wingfield BD, Wingfield MJ (2011) Occurrence and pathogenicity of Neofusicoccum parvum and N. mangiferae on ornamental Tibouchina species. For Path 41:48-51. doi: 10.1111/j.1439-0329.2009.00635.x

Jakhetia V, Patel R, Khatri P, Pahuja N, Garg S, Pandey A, Sharma S (2010) Cinnamon: A pharmacological review. J Adv Sci Res 1:19-23

Kusari S, Hertweck C, Spiteller M (2012) Chemical ecology of endophytic fungi: Origins of secondary metabolites. Chem Biol 19:792-798. doi: 10.1016/j.chembiol.2012.06.004

Lopes A, Barradas C, Phillips AJL, Alves A (2016) Diversity and phylogeny of Neofusicoccum species occurring in forest and urban environments in Portugal. Mycospere 7:906-920.  doi: 10.5943/mycosphere/si/1b/10

Maehara S, Ikeda M, Haraguchi H, Kitamura C, Nagoe T, Ohashi K, Shibuya H (2011) Microbial conversion of curcumin into colorless hydroderivatives by the endophytic fungus Diaporthe sp. associated with Curcuma longa. Chem Pharm Bull 59:1042-1044. doi: 10.1248/cpb. 59.1042

Molyneux P (2004) The use of the stable free radical diphenylpicryl-hydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J Sci Technol 26:211-219

Praptiwi, Ilyas M, Wulansari D,  Agusta A (2015) Antibacterial screening of the culture of endophytic fungal extracts isolated from cinnamon stick (Cinnamomum burmanni [Nees & T. Nees] Blume). J Teknologi Indones 38:33-41. doi: 10.14203/jti.v38i1.159

Priani SE, Darusman F, Humanisya H (2014) Formulasi sediaan emulgel antioksidan mengandung ekstrak etanol kulit batang kayu manis (Cinnamomum burmanni Nees Ex. Bl.). Prosiding SNaPP 2014 Sains, Teknologi dan Kesehatan 4:103-110

Putri IJ, Fauziyah, Elfita (2013) Aktivitas antioksidan daun dan biji buah nipah (Nypa fruticans) asal pesisir Banyuasin Sumatera Selatan dengan metode DPPH. Maspari J 5:16-21

Saxena M, Saxena J, Nema R, Singh D, Gupta A (2013) Phytochemistry of medicinal plants. J Pharmacogn Phytochem 1:168-182

Slippers B, Wingfield MJ (2007) Botryosphaeriaceae as endophytes and latent pathogens of woody plants: diversity, ecology and impact. Fungal Biol Rev 21:90-106. doi: 10.1016/ j.fbr.2007.06.002

Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731-2739. doi: 10.1093/molbev/msr121

White TJ, Bruns T, Lee S, Taylor I (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. Pp 315-322. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, (Eds). PCR Protocols: A Guide to Method and Applications. Academic Press, New York

Widowati T, Bustanussalam, Sukiman H, Simanjuntak P (2016) Isolasi dan identifikasi kapang endofit dari tanaman kunyit (Curcuma longa L.) sebagai penghasil antioksidan. Biopropal Indust 7:9-16




DOI: http://dx.doi.org/10.29122/jbbi.v5i2.3052

Refbacks

  • There are currently no refbacks.


© Copyright: BY-NC-SA