PROFIL HEMATOLOGI TIKUS (<em>Rattus norvegicus</em>) GALUR <em>SPRAGUE-DAWLEY</em> JANTAN UMUR 7 DAN 10 MINGGU

Main Article Content

Idah Rosidah
Sri Ningsih
Tiya Novita Renggani
Julham Efendi
Kurnia Agustini

Abstract

Hematology Profile of Sprague-Dawley Male Rats (Rattus norvegicus) Aged 7 and 10 Weeks

Currently, the normal hematology profile has not been yet available for outbred rats in Indonesia. Most of the researchers use the data of hematology profile from references outside Indonesia in which the breeding environment is different. This study aimed to obtain a hematology profile of Sprague-Dawley male rats aged 7 and 10 weeks. The animals were obtained from the Laboratory of Animal Experiment, NADFC. Blood was drawn from the retro-orbital sinus and then analyzed to obtain the hematology profile including erythrocytes, hemoglobin, hematocrit, MCV, MCH, MCHC, leukocytes. Statistically significant differences for erythrocytes, hemoglobin, MCV, MCHC, thrombocyte, and leukocytes parameters were observed between rats aged 7 and 10 weeks. However, there were no statistically significant differences observed on MCH, thrombocyte, and body weight parameters. Data presented here would be useful as a reference for hematology research as well as in evaluating alterations in blood parameters.

Keywords: erythrocyte, hematology, leukocyte, Sprague-Dawley, thrombocyte

ABSTRAK

Saat ini belum banyak tersedia informasi profil hematologi untuk hewan coba tikus yang dikembangbiakkan di Indonesia. Kebanyakan peneliti menggunakan data profil hematologi dari referensi luar Indonesia. Penelitian ini bertujuan untuk mendapatkan data hematologi tikus jantan usia 7 dan 10 minggu.  Hewan coba yang digunakan adalah tikus jantan galur Sprague-Dawley yang diperoleh dari Laboratorium Hewan Percobaan-BPOM. Sebanyak 60 ekor tikus jantan yang terdiri dari 30 ekor usia 7 minggu dan 30 ekor usia 10 minggu diambil darahnya melalui sinus orbitalis. Selanjutnya, dianalisa jumlah sel eritrosit, hemoglobin, hematokrit, MCV, MCH, MCHC, leukosit. Hasil analisis statistik menunjukkan terdapat perbedaan signifikan antara tikus jantan usia 7 dan 10 minggu untuk parameter jumlah eritrosit, hemoglobin, hematokrit, MCV, MCHC dan leukosit. Tetapi tidak terdapat perbedaan signifikan untuk jumlah MCH, trombosit dan berat badan tikus. Data yang diperoleh dapat digunakan sebagai referensi penelitian yang berhubungan erat dengan profil hematologi dan penyakit kelainan darah lainnya.

Article Details

How to Cite
Rosidah, I., Ningsih, S., Renggani, T. N., Efendi, J., & Agustini, K. (2020). PROFIL HEMATOLOGI TIKUS (<em>Rattus norvegicus</em>) GALUR <em>SPRAGUE-DAWLEY</em> JANTAN UMUR 7 DAN 10 MINGGU. Jurnal Bioteknologi & Biosains Indonesia (JBBI), 7(1), 136–145. https://doi.org/10.29122/jbbi.v7i1.3568
Section
Research Articles

References

Andersen ML, e Costa RM, e Costa MFO (2016)  Rats. In: Andersen ML, Tufik S (Eds). Rodent model as tools in ethical biomedical research. Springer International Pub Switzerland, pp 61–64. doi: 10.1007/978-3-319-11578-8_7

Andreollo NA, Santos EF, Araújo MR, Lopes LR (2012) Rat's age versus human's age: what is the relationship? Arq Bras Cir Dig. 25:49–51. doi:10.1590/s0102-67202012000100011

Bailly Y, Duprat P (1990) Normal blood cell values, rat. In: Jones  TC, Ward JM, Mohr U, Hunt RD (Eds). Hemopoietic system. Monographs on pathology of laboratory animals. Springer, Berlin, pp 27–38. doi: 10.1007/978-3-642-84110-1_3

Baumans V (2016) The aspects of the use of rodents in experimental research. In: Andersen ML, Tufik S (Eds). Rodent model as tools in ethical biomedical research. Springer International Pub Switzerland,  pp 7–12. doi: 10.1007/978-3-319-11578-8_2

Boone CD, Gill S, Habibzadegan A, Robert McKenna (2013) Carbonic anhydrase: An efficient enzyme with possible global implications. Int J Chem Eng 2013:1–6. ID 813931. doi: 10.1155/2013/813931

BPOM (2014) Peraturan Kepala Badan Pengawas Obat dan Makanan Republik Indonesia Nomor 7 tahun 2014 tentang Pedoman uji toksisitas nonklinik secara in vivo. Badan Pengawas Obat dan Makanan Republik Indonesia, Jakarta

Car BD, Eng VM, Everds NE, Bounous DI (2006) Clinical pathology of the rat. In: Suckow M, Weisbroth S, Franklin C (Eds). The laboratory rat. 2nd Edition.  American College of Laboratory, Animal Medicine Series. Academic Press, Elsever Inc, New York, pp 127–146. doi: 10.1016/B978-012074903-4/50008-X

Cora MC, King D, Betz LJ, Wilson R, Travlos GS (2012) Artifactual changes in Sprague-Dawley rat hematologic parameters after storage of samples at 3ºC and 21ºC. J Am Assoc Lab Anim Sci 51:616–621. PMID: 23312091

Delwatta SL, Gunatilake M, Baumans V, Seneviratne MD, Dissanayaka MLB, Batagoda SS, Udagedara AH, Walpola PB (2018) Reference values for selected hematological, biochemical and physiological parameters of Sprague-Dawley rats at the Animal House, Faculty of Medicine, University of Colombo, Sri Lanka. Anim Model Exp Med 1:1–5. doi: 10.1002/ame2.12041

Fitria L, Sarto M (2014) Profil hematologi tikus (Rattus norvegicus Berkenhout, 1769) galur wistar jantan dan betina umur 4, 6, dan 8 minggu. Biogenesis 2:94–100. doi: 10.24252/bio.v2i2.473

Fitria L, Illiy LL, Dewi IR (2016) Pengaruh antikoagulan dan waktu penyimpanan terhadap profil hematologis tikus (Rattus norvegicus Berkenhout, 1769) galur wistar. Biosfera 33:22–30. doi: 10.20884/1.mib.2016.33.1.321

Thomas C, Lumb AB (2012) Physiology of haemoglobin. BJA Education 12:251–256. doi: 10.1093/bjaceaccp/mks025

Giknis MLA, Clifford CB (2006) Clinical laboratory parameters for Crl:CD(SD) rats. Charles River Lab, Wilmington USA

Gileta AF, Fitzpatrick CJ, Chitre AS, St Pierre CL, Joyce EV, Maguire RJ, McLeod AM, Gonzales NM, Williams AE, Morrow JD, Robinson TE, Flagel SB, Palmer AA (2018) Genetic characterization of outbred Sprague Dawley rats and utility for genome-wide association studies. BioRxiv 412924. doi: 10.1101/412924

He Q, Su G, Liu K, Zhang F, Jiang Y, Gao J, Liu L, Jiang Z, Jin M, Xie H (2017) Sex-specific reference intervals of hematologic and biochemical analytes in Sprague-Dawley rats using the nonparametric rank percentile method. PLoS One 12:e0189837. doi: 10.1371/journal.pone.0189837

Holinstat M (2017) Normal platelet function. Cancer metastasis reviews 36:195–198. doi: 10.1007/s10555-017-9677-x

Ihedioha JI, Okafor C, Ihedioha TE (2004) The haematological profile of the Sprague-Dawley outbred albino rat in Nsukka, Nigeria. Anim Res Int 1:125–132. doi: 10.4314/ari.v1i2.40755

Johnson M (2012) Laboratory mice and rats. Mater Methods 2012 2:113. doi: 10.13070/mm.en.2.113

Kartika D, Dewi FNA, Iskandriati D, Winoto I, Permanawati, Narani A, Suhartin, Budiarsa IN (2014) Nilai parameter hematologi tikus Sprague Dawley dan mencit Balb/C di Indonesia sebagai referensi dalam penelitian biomedis. HL-03, pp 109-111. Pros Konferensi Ilm Vet Nas (KIVNAS) ke-13, 23–26 November 2014, Palembang

Lillie LE, Temple NJ, Florence LZ (1996) Reference values for young normal Sprague-Dawley rats: Weight gain, hematology and clinical chemistry. Hum Exp Toxicol 15:612–616. doi: 10.1177/096032719601500802

Lindstrom NM, Moore DM, Zimmerman K, Smith SA (2015) Hematologic assessment in pet rats, mice, hamsters, and gerbils: Blood sample collection and blood cell identification. Clin Lab Med 35:629–640. doi: 10.1016/j.cll.2015.05.011

NRC (2011) Guide for the care and use of laboratory animals, 8th Edition. National Research Council, Institute for Laboratory Animal Research. The National Academies Press, Washington DC. doi: 10.17226/12910

Parasuraman S, Raveendran R, Kesavan R (2010) Blood sample collection in small laboratory animals. J Pharmacol Pharmacother 1:87–93. doi:10.4103/0976-500X.72350

Reece WO, Rowe EW (2017) Functional anatomy and physiology of domestic animals, 5th Edition. Wiley Blackwell, New York

Said NM, Abiola O (2014) Haematological profile shows that inbred Sprague Dawley rats have exceptional promise for use in biomedical and pharmacological studies. Asian J Biomed Pharm Sci 4:33–37. doi: 10.15272/ajbps.v4i37.597

Smith C, Jarecki A (2011) Atlas of comparative diagnostic and experimental hematology. 2nd Edition. Wiley-Blackwell, Hong Kong. doi: 10.1002/9781118785072

Vandamme TF (2014) Use of rodents as models of human diseases. J Pharm Bioallied Sci 6:2–9. doi: 10.4103/0975-7406.124301

Wolford ST, Schroer RA, Gallo PP, Gohs FX, Brodeck M, Falk HB, Ruhren R (1987) Age-related changes in serum chemistry and hematology values in normal Sprague-Dawley rats. Toxicol Sci 8:80–88. doi: 10.1093/toxsci/8.1.80