PERBANDINGAN AKTIVITAS ANTIBAKTERI EKSTRAK KASAR RESIN DAN DAUN GAHARU (<em>Gyrinops versteegii</em>)

Main Article Content

I Gde Adi Suryawan Wangiyana
. Akram
Farouq Isbulloh

Abstract

Comparison of Antibacterial Activities of Agarwood (Gyrinops versteegii) Resin and Leaf Crude Extract

This research aim is to compare antibacterial activities of leaves extract and resin extract of Gyrinops versteegii with different extraction solvent and concentration. Leaves and resin had been prepared by drying and grinding then were extracted by maceration method. Factorial experiment design was used for extract application to Staphylococcus aureus ATCC25923. First factor was extract ingredient, second factor was extraction solvent, third factor was extract concentration. Inhibition zone as main parameter for antibacterial assay were analysed by ANOVA, HSD test and standard error. The inhibition zone of resin was higher than inhibition zone of leaves. The extraction solvent and extract concentration were not significantly resulted in different inhibition zone diameter. However, there were unique interaction between extraction solvent and extraction concentration that affected inhibition zone diameter. It could be concluded that the inhibition zone of resin was higher than that of leaves while no significant result from extraction solvent and extract concentration factors

Keywords: antibacterial, agarwood, extract, leaves, resin

 

ABSTRAK

Penelitian ini bertujuan untuk membandingkan aktivitas antibakteri daun gaharu dan resin gaharu G. versteegii menggunakan beberapa pelarut pengekstrak dan konsentrasi ekstrak yang berbeda. Bahan daun dan resin gaharu dikeringkan dan dicacah, kemudian diekstrak menggunakan metode maserasi. Uji aplikasi ekstrak pada Staphylococcus aureus ATCC25923 dilakukan menggunakan rancangan faktorial, yaitu faktor pertama bahan ekstrak (resin, daun), faktor kedua pelarut pengekstrak (etanol, metanol), dan faktor ketiga konsentrasi ekstrak (0,25; 0,5; dan 1 g mL–1). Data zona hambat terhadap bakteri uji dianalisis menggunakan ANOVA, BNJ dan standard error. Rerata zona hambat ekstrak daun gaharu (7 mm) lebih besar secara signifikan dibandingkan dengan rerata zona hambat ekstrak resin (6,5 mm). Faktor pelarut pengekstrak dan konsentrasi ekstrak tidak berpengaruh signifikan terhadap zona hambat. Pelarut pengekstrak dan konsentrasi ekstrak memberikan pengaruh interaksi berbeda-beda terhadap zona hambat. Dapat disimpulkan bahwa bahan ekstrak daun gaharu memiliki aktivitas antibakteri lebih bagus dibandingkan dengan ekstrak resin gaharu dengan konsentrasi ekstrak efisien sebesar 0,25 g mL–1.

Article Details

How to Cite
Wangiyana, I. G. A. S., Akram, ., & Isbulloh, F. (2020). PERBANDINGAN AKTIVITAS ANTIBAKTERI EKSTRAK KASAR RESIN DAN DAUN GAHARU (<em>Gyrinops versteegii</em>). Jurnal Bioteknologi & Biosains Indonesia (JBBI), 7(1), 28–36. https://doi.org/10.29122/jbbi.v7i1.3862
Section
Research Articles
Author Biography

I Gde Adi Suryawan Wangiyana, Program Studi Kehutanan, Universitas Pendidikan Mandalika, Jl Pemuda No 59A Dasan Agung Baru, Mataram

Kehutanan

References

Ahmad ZA, Yusoff Z, Awang AF, Mohd Nor Rudin MAF, Mohd Zait MSH, Roslan MH, Mat Zaid MZI (2015) Hydro-distillation process in extracting of agarwood essential oil. In: Abd. Wahab Dul Ahad NB, Said NAB, Razali NB, Atan SB, Mat Salleh NB (eds) Technology and Innovation National Conference Proceedings (TECHON 2015). Politeknik Mukah, Serawak, pp 203–211 doi: 10.13140/RG.2.1.2091.0168

Altman DG, Bland JM (2005) Statistic notes: Standard deviation and standard errors. Br Med J 331:903. doi:  10.1136/bmj.331.7521.903

Anggadhania L, Anita Nugraheni YMM, Wangiyana IGAS, Nawawi M, Soetarto ES (2019) Biomass enhancement of agarwood formation on Gyrinops versteegii (Gilg.) Domke in Lombok. Int J Sustain Biomass Bioenerg 2:1–5

Appiah T, Boakye YD, Agyare C (2017) Antimicrobial activities and time-kill kinetics of extract of selected Ghanaian mushrooms. Evid Based Complement Alternat Med 2017:4534350. doi:  10.1155/2017/4534350

Arifin S, Bhuiyan MZI (2019) Antagonistic activity of Bacillus amyloliquefaciens subsp. amyloliquefaciens against multidrug resistant Serratia rubidaea. BioRxiv the Reprint Server for Biology. doi: 10.1101/818054

Azwanida NN (2015) A Review on the extraction methods use in medicinal plants, principle, strength and limitation. Med Aromat Plants 4:1000196. doi: 10.4172/2167-0412.1000196

Camargo TCD, Graziano KU, Almeida AGCDS, Suzuki K, da Silva CB, Pinto FMG (2016) Microbiological evaluation of the steam sterilization of assembled laparoscopic instruments. Rev Lat Am Enfermagem 24:e2830. doi: 10.1590/1518-8345.1431.2830

Chen H, Yang Y, Xue J, Wei J, Zhang Z, Chen H (2011) Comparison of compositions and antimicrobial activities of essential oils from chemically stimulated agarwood, wild agarwood and healthy Aquilaria sinensis (Lour.) Gilg trees. Molecule 16:4884–4896. doi: 10.3390/molecules16064884

Febrina L, Rusli R, Muflihah F (2015) Optimalisasi ekstraksi dan uji metabolit sekunder tumbuhan libo (Ficus variegate Blume). J Trop Pharm Chem 3:74–81. doi: 10.25026/jtpc.v3i2.153 

Hasim YZ, Kerr PG, Abbas P, Mohd Salleh H (2016) Aquilaria spp. (agarwood) as source of health beneficial compounds: A review of traditional use, phytochemistry and pharmacology. J Ethnopharmacol 189:331–360. doi: 10.1016/j.jep.2016.06.055

Iswantari W, Mulyaningish T, Muspiah A (2017) Karyomorfologi dan jumlah kromosom empat grup Gyrinops versteegii (Gilg.) Domke. di Lombok. J Ilmu Kehutan 11:205–211. doi: 10.22146/jik.28284

Iqbal J, Siddiqui R, Kazmi SU, Kahn NA (2013) A simple assay to screen antimicrobial compounds potentiating the activity of current antibiotics. BioMed Res Int 2013:927323. doi: 10.1155/2013/927323

Jok VA, Che Radzi N, Ku Hamid KH (2015) A review: Pharmacological properties of Aquilaria spp. Adv Mater Res 1113:193–197. doi: 10.4028/www.scientific.net/AMR.1113.193

Kakino M, Sugiyama T, Kunieda H, Tazawa S, Maruyama H, Tsuruma K, Araki Y, Shimazawa M, Ichihara K, Mori H, Hara H (2012) Agarwood (Aquilaria crassna) extracts decrease high-protein high-fat diet-induced intestinal putrefaction toxins in mice. Pharm Anal Acta 3:1000152. doi: 10.4172/2153-2435.1000152

Kamonwannasit S, Nantapong N, Kumkrai P, Luecha P, Kupittayanant S, Chudapongse N (2013) Antibacterial activity of Aquilaria crassna leaf extract against Staphylococcus epidermidis by disruption of cell wall. Ann Clin Microbiol Antimicrob 12:20. doi: 10.1186/1476-0711-12-20

Kim HY (2014) Statistical notes for clinical researchers: Two-way analysis of variance (ANOVA)-exploring possible interaction between factors. Restor Dent Endod 39:143–147. doi: 10.5395/rde.2014.39.2.143

Kristanti AN, Tanjung M, Aminah NS (2018) Review: Secondary metabolites of Aquilaria, a Thymelaeaceae Genus. Mini Rev Org Chem. 15:36–55. doi: 10.2174/1570193X14666170721143041

Saquib SA, Al-Qahtani NA, Ahmad I, Kader MA, Al Shahrani SS, Asiri EA (2019) Evaluation and comparison of antibacterial efficacy of herbal extract in combination with antibiotics on periodontal pathobionts: An in vitro microbiological study. Antibiotics 8:E89. doi: 10.3390/antibiotics8030089

Silva E, Fernandes S, Bacelar E, Sampaio A (2016) Antimicrobial activity of aqueous, ethanolic and methanolic leaf extracts from Acacia spp. and Eucalyptus nicholii. Afr J Tradit Complement Altern Med 13:130–134. doi: 10.21010/ajtcam.v13i6.18

Tan CS, Isa NM, Ismail I, Zainal Z (2019) Agarwood induction: current developments and future perspectives. Front Plant Sci 10:122. doi: 10.3389/fpls.2019.00122

Turjaman M, Hidayat A, Santoso E (2016) Development of agarwood induction technology using endophytic fungi. In: Mohamed R (ed) Agarwood: Tropical forestry – Science behind the fragrance. Springer, Singapore, pp 57–71. doi: 10.1007/978-981-10-0833-7_4

Thouri A, Chahdoura H, El Arem A, Hichri AO, Hassin RB, Achour L (2017) Effect of solvents extraction on phytochemical components and biological activities of Tunisian date seeds (var. Korkobbi and Arechti). BMC Complement Altern Med 17:248. doi:  10.1186/s12906-017-1751-y

Wang S, Yu Z, Wang C, Wu C, Guo P, Wei J (2018) Chemical constituent and pharmacological activity of agarwood and Aquilaria plants. Molecules 23:342. doi: 10.3390/molecules23020342 

Wangiyana IGAS, Malik S (2018) Application of arbuscular mycorrhiza from Senaru Forest rhizosphere for Gyrinops versteegii germination and growth. Biosaintifika 10:432–438. doi: 10.15294/biosaintifika.v10i2.14396

Wangiyana IGAS, Sami’un (2018) Characteristic of agarwood tea from Gyrinops versteegii fresh and dry leaves. J Sangkareang Mataram 4:41–44

Wangiyana IGAS, Sawaludin, Nizar WY, Wangiyana W (2019) Tannin concentrations of Gyrinops tea with different leaf processing methods and addition of herbal medicine ingredients. AIP Conf Proc 2199: 070012. doi: 10.1063/1.5141326

Wangiyana IGAS, Wanitaningsih SK (2018) Pembuatan inokulan gaharu berbasis bahan baku tauge untuk masyarakat Desa Pejaring Timur.  J Abdi Insani 5:85–91

Wolde T, Kuma H, Trueha K, Yabeker A (2018) Anti-bacterial activity of garlic extract against human pathogenic bacteria. J Pharmacovigil 6:1000253. doi: 10.4172/2329-6887.1000253

Zhang Z, Han X, Wei J, Xue J, Yang Y, Liang L, Li X, Guo Q, Xu Y, Gao Z (2014) Compositions and antifungal activities of essential oils from agarwood of Aquilaria sinensis (Lour.) Gilg induced by Lasiodiplodia theobromae (Pat.) Griffon. & Maubl. J Braz Chem Soc 25:20–26. doi: 10.5935/0103-5053.20130263