THE COMBINATION OF GROWTH HORMONES INCREASED THE IN VITRO SHOOTS MULTIPLICATION ON SAGO PALM (Metroxylon sagu Rottb.)

Teuku Tajuddin, Karyanti ., Tati Sukarnih, Nadirman Haska

Abstract


Pohon sagu (Metroxylon sagu Rottb.) mempunyai banyak keunggulan dibanding dengan tanaman-tanaman penghasil pati lainnya, khususnya karena memiliki produktivitas yang tinggi, tumbuh di area bantaran sungai dan rawa, yang merupakan lingkungan tidak sesuai bagi pertumbuhan tanaman-tanaman lain. Dalam rangka membangun suatu perkebunan sagu di area yang luas, maka sangat dibutuhkan anakan-anakan sagu yang ukurannya seragam dalam jumlah yang besar. Namun demikian, terbatasnya jumlah anakan yang seragam telah menjadi kendala bagi pengembangan perkebunan sagu. Sebagai alternatif, perbanyakan in vitro dengan induksi tunas langsung dilakukan untuk mendapatkan bibit-bibit sagu dengan genotip unggul secara masal. Anakan sagu yang diperoleh dari Propinsi Maluku digunakan sebagai sumber eksplan. Eksplan dikultur pada media MS dan B5 yang mengandung kombinasi hormon auksin dan sitokinin. Hasil penelitian menunjukkan bahwa perlakuan BAP 2.0 ppm dan NAA 2.0 ppm menghasilkan jumlah tunas terbanyak.

Kata kunci: Auksin, sitokinin, in vitro, sagu, inisiasi tunas

 

ABSTRACT

Sago palm (Metroxylon sagu Rottb.) has many advantages over other starch-producing crops especially for its higher yield, ability to grow along riverbanks and on swampy areas not suitable for other crops. With the purpose of establishing large-scale plantations, a large amount of uniform sago palm suckers are required. However, limited availability of uniform suckers has hindered the mass propagation and development of cultivated Sago palm. Alternatively, in vitro cultures were performed in order to obtain a large-scale of mass clonally propagation of superior genotypes of sago palm. The young suckers obtained from areas of Maluku Province were used as explants. In vitro culture was carried out through direct shooting. The explants were cultured on two kinds of media, which were MS and B5 media containing various growth hormones of auxins and cytokinins. The results showed that the treatment with BAP 2.0 ppm and NAA 2.0 ppm produced the highest number of shoots.

Keywords: Auxin, cytokinin, in vitro, sago palm, shoot initiation

Full Text:

PDF

References


Alang ZC, Krishnapillay B, Pilus SF, Hisajima S (1991) Embryo culture and nursery establishment of sago palm (Metroxy-lon spp.) plantlets. Proceedings of the Fourth International Sago Symposium, August 6-9, 1990, Kuching, Serawak, Malaysia

Flach M (1997) Sago Palm, Metroxylon sagu Rottb. Promoting the Conservation and use of Underutilized and Neglected Crops, 13. International Plant Genetic Resource Institute (IPGRI) Italy and IPK Germany, 71pp

Gamborg OL, Murashige T, Thorpe TA, Vasil IK (1976) Plant tissue cultura media. In Vitro 12:473-478

Handley LW (1995) Future uses of somatic embryogenesis in woody plantation species. In:S. Jain (ed) Somatic Embryogenesis in Woody Plants Vol 1:415-434

Hisajima S, Jong FS, Arai Y, Sim ES (1991) Propagation and breeding of sago palm (Metroxylon sagu Rottb.) plant in vitro:1. Embryo culture and induction of multiple shoots from sago embryos in vitro. Jap J Trop Agric 35:259-267

Jong FS (1995) Research for the development of sago palm (Metroxylon sagu Rottb.) cultivation in Sarawak, Malaysia. PhD Thesis, Wageningen University 139pp.

Kasi PD, Sumaryono (2006) Keragaman morfologi selama perkembangan embrio somatik sagu (Metroxylon sagu Rottb). Menara Perkebunan 74:44-52

Miftahorrachman, Novarianto H (2003) Jenis-jenis sagu potensial di Sentani Irian Jaya. Dalam Prosiding Seminar Ketahanan Pangan. Manado 6 Oktober 2003

Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473-497

Okazaki M, Tadenuma S, Ohmi M (2005) Diverse utilization and industrial development of sago biomass. Proceeding Abstract p 2. In YP Karafir, VE Fere and Y Toyoda (Eds). The Eight International Sago Symposium, August 4-6, 2005, Manokwari. The Japan Society for Promotion of Science

Riyadi I, Tahardi JS, Sumaryono (2005) The development of somatic embryos of sago palm (Metroxylon sagu Rottb) on solid media. Menara Perkebunan 73:35-43

Setyabudi A, Widyaningtyas N (2008) Sagu (Metroxylon spp.) sebagai Sumber Energi Bioetanol Potensial. Badan Penelitian dan Pengembangan Kehutanan Departemen Kehutanan

Sumaryono, Riyadi I, Kasi PD (2007) Perbanyakan klonal tanaman sagu unggul melalui kultur jaringan. Prosiding Lokakarya Pengembangan Sagu di Indonesia. Pusat Litbang Perkebunan, Batam 25-26 Juli 2007

Sumaryono, Riyadi I, Kasi PD (2009) Clonal propagation of sago palm (Metroxylon sagu Rottb) through tissue culture. J Appl Ind Biotechnol Trop Reg 2:1-4

Sumaryono, Muslihatin W, Ratnadewi D (2012) Effect of carbohydrate source on growth and performance of in vitro sago palm (Metroxylon sagu Rottb) plantlets. Hayati J Biosci 19:88-92

Tahardi JS, Sianipar NF, Riyadi I (2002) Somatic embryogenesis in sago palm (Metroxylon sagu Rottb.). In:K Kaimuna, M Okazaki, Y Toyoda, JE Cecil (eds) New Frontiers of Sago Palm Studies p75-81. Universal Academic Press, Inc. Tokyo, Japan

Tajuddin T, Karyanti, Sukarnih T, Haska N (2014) A revised method for sago palm (Metroxylon sagu Rottb.) sucker explants sterilization to support the in vitro propagation. J Bioteknol Biosains Indones 1:21-26

Yusnita (2004) Kultur Jaringan Cara Memperbanyak Tanaman secara Efisien. Agromedia Pustaka. Jakarta




DOI: http://dx.doi.org/10.29122/jbbi.v2i1.532

Refbacks

  • There are currently no refbacks.


© Copyright: BY-NC-SA