MEKANISME PELEPASAN HORMON GONADOTROPIN (GtH-II) IKAN LELE (Clarias sp) SETELAH DI INDUKSI LASERPUNKTUR PADA TITIK REPRODUKSI

kusuma P.S.W, Agung P.W. Marhendra, Aulanni’am Aulanni’am

Abstract


Induction laserpuncture effectively stimulates gonad maturation and spawning, but the associated gonadotropin releasing hormone (LH / GtH-II) mechanism is still unknown. So the purpose of this study demonstrate the mechanism of the release of GTH-II on catfish (Clarias sp.) after induction laserpuncture at the reproduction acupoint. The test fish consisted of 54 males and 54 females aged 8-9 months from the F1 hybrid Sangkuriang type female and Paiton type male parents. Our
study employed an experimental method with completely randomized design. The treatment comprised 6 levels with 9 repetitions. We were to observe gonadotropin hormone (GtH-II) concentrations pre-spawn, spawn, post-spawning in the laserpuncture exposed. As a comparison investigations were also conducted to untreated (control). Blood sampling performed at 6 h after induction laserpuncture. Hormone levels using the Elisa test kits Fish Luteinizing Hormone (LH) BIOTECH CO. CUSABIO., LTD. by Catalog No.. CSB-E15791Fh. The test results significant effect (P<0.05) increased levels of GtH-II profiles between laserpuncture induction
with control, which profiles GTH-II levels pre-spawn conditions higher 2.2468, as well as the higher spawn condition 1,5409 in comparison with controls. However, the condition post-spawning in induction laserpuncture lower value 1.1131 compared with controls. This suggests that induction laserpuncture at the reproduction acupoint can stimulate of GABA release from GABAergic neurons pre-spawn and spawn condition. GABA stimulates the hypothalamus to release GnRH neurons. GnRH neurons stimulates the pituitary to GtH-II release. GtH-II will affect the acceleration of gonad maturation and spawning.

Full Text:

PDF


DOI: https://doi.org/10.29122/jsti.v14i3.928

Refbacks

  • There are currently no refbacks.