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Vegetative propagation from bulb excised of Eleutherine sp. (Iridaceae) were culturedÂ in Murashige and Skoog (MS) medium supplemented with plant growth regulatorÂ (PGR) Benzyl adenine (BA) 1 mg/l at initiation stage, BA (2 and 5) mg/l for inducedÂ shoot buds formation at multiplication stage. In this study also BA 2 mg/l and BA 2 mg/lÂ combined with naphthalein acetic acid (NAA) 0.5 mg/l were treated for rooting planletsÂ formation. Calli formation were induced with auxin PGR picloram 1 mg/l combined withÂ 2,4 dichlorophenoxy acetic acid (2,4-D) 0.5 and 1 mg/l in concentrations. The mediaÂ contained 2 mg/l cytokinin (BA) without auxin (NAA), resulted the highest shoot budsÂ formation. Rooting planlets were produced in MS medium combined with BA and NAA.MS medium contains Picloram 1 mg/l and 2,4-D 1 mg/l was optimal for frequency on
calli initiation (100%) and the largest diameter of calli also represented in cultured MSÂ medium with picloram 1 mg/l and 2,4-D 1mg/l. In acclimatization stage, 100% of planletsÂ survived and successfully transplanted to soil medium in the field.
Key words: in vitro, Eleutherine sp.
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