AKTIVITAS AKTINOMISETES DARI BANGKA-BELITUNG KOLEKSI BIDANG MIKROBIOLOGI, PUSLIT BIOLOGI- LIPI DALAM MEMPRODUKSI ENZIM KITINASE

Main Article Content

yati Sudaryati Soeka
Evi Triana
Ninu Setianingrum

Abstract

The aim of the research was to know the capability of actinomycetes isolate from Bangka Belitung, which stored at Microbiology-LIPI Culture Collection, in producing chitinase enzyme. This isolate which could produce chitinolitic enzyme, signed by clear zone at medium contain 1% chitine. The chitinase activity of the isolate which incubated for 1-7 days in the room temperature was analyzed by spectrophotometer in λ 584 nm. The result of this experiment was highest chitinase activities with incubated for 3 days, were 1.66 . 10-2 U/ml. Maximum chitinase activities was found at 1% starch soluble substrate 2.83 . 10-2, pH 8.0 and at 50°C condition were 9.3 . 10-2 and 12.98 .10-2 U/ml respectively.
Key words : chitinase, clear zone, spectrophotometer

Article Details

Section
RESEARCH ARTICLES
Author Biographies

yati Sudaryati Soeka, Lembaga Ilmu Pengetahuan Indonesia

Peneliti di Bidang Mikrobiologi, Puslit Biologi- LIPI, Cibinong

Evi Triana, Lembaga Ilmu Pengetahuan Indonesia

Peneliti di Bidang Mikrobiologi, Puslit Biologi- LIPI, Cibinong

Ninu Setianingrum, Lembaga Ilmu Pengetahuan Indonesia

Peneliti di Bidang Mikrobiologi, Puslit Biologi- LIPI, Cibinong