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from Halimeda opuntia, whereas Hydroclathrus clathratus produced 6 compounds (4Â fatty acids, one compound each of steroid and ether). All isolated single compounds
were tested for their antibacterial activities by the agar diffusion method against theÂ Gram-positive bacteria Staphylococcus aureus, Bacillus subtilis and Streptococcus
faecalis, and the Gram-negative bacteria Echerichia coli, Pseudomonas aeruginosaÂ and Salmonella typhimurium. All 14 compounds showed activity against Gram-positive
bacteria, especially Bacillus subtilis, and only 2 compounds showed activity againstÂ Gram-negative bacteria Escherichia coli. Nine compounds showed activity againstÂ Staphylococcus aureus, and 4 compounds showed activity against Streptococcus
faecalis. All compounds were not active against Pseudomonas aeruginosa and
Salmonella typhimurium bacteria. This study indicated that there is indeed a diversity
both in kinds and in molecular structures of the antibacterial substances.
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