Main Article Content

Bedah Rupaedah
Yuichiro Takahashi


Pengaruh Suplai Nitrogen pada Media Kultur dan Intensitas Cahaya Terhadap Proses Fotosintesis Chlamydomonas reinhardtii

Organisms use nitrogen to produce, among others, amino acids, proteins, and nucleic acids. In this study, the effects of various concentrations of ammonium in culture media on the photosynthetic performance of Chlamydomonas reinhardtii were done under two light conditions: low and high intensity. The microbes were grown at low (75% NH4Cl dosage), normal (100% NH4Cl dosage, which was 2 M NH4Cl), and high (125% NH4Cl dosage) nitrogen content. Cells density and chlorophyll content were quantitatively determined. Immunoblotting technique was used to separate proteins based on molecular mass. In both low and high light intensity, cells grown in 75% NH4Cl dosage culture medium showed lower cell density and chlorophyll concentration than those grown in 100% and 125% NH4Cl dosage media. The later two media produced almost the same amount of cell density and chlorophyll concentration. In conclusion, 75% NH4Cl dosage was insufficient for C. reinhardtii cells to grow well. The results also showed that accumulation of photosystem I (PsaA and PsaD/F) and light harvesting complex II (LHCII) were higher in low light than in high light intensity.


Organisme menggunakan nitrogen diantaranya untuk memproduksi asam amino, protein, dan asam nukleat. Dalam percobaan ini pengaruh berbagai konsentrasi amonium dalam media pada fotosintesis Chlamydomonas reinhardtii dilakukan di bawah dua kondisi cahaya: intensitas rendah dan tinggi. C. reinhardtii ditumbuhkan dalam medium dengan dosis nitrogen (N) rendah (75% dosis NH4Cl), normal (100% dosis NH4Cl, yakni NH4Cl 2 M), dan tinggi (125% dosis NH4Cl). Parameter yang diukur adalah  kepadatan sel dan konsentrasi klorofil. Analisis protein dilakukan dengan imunobloting untuk memisahkan protein berdasarkan massa molekul. Pada intensitas cahaya rendah dan tinggi, sel-sel pada medium dengan 75% NH4Cl menunjukkan kepadatan sel dan konsentrasi klorofil lebih rendah dibandingkan dengan 100% NH4Cl dan 125% NH4Cl, di mana kedua media ini menghasilkan kepadatan sel maupun konsentrasi klorofil yang hampir sama. Dengan demikian dapat disimpulkan bahwa 75% NH4Cl tidak cukup bagi C. reinhardtii untuk tumbuh dengan baik. Selain itu, akumulasi fotosistem I (PsaA dan PsaD/F) dan kompleks pemanenan cahaya II (LHCII) lebih tinggi pada sistem fotosintesis dengan intensitas cahaya rendah dibandingkan cahaya tinggi.

Kata kunci: Chlamydomonas reinhardtii, fotosintesis, intensitas cahaya, klorofil, nitrogen

Article Details

How to Cite
Rupaedah, B., & Takahashi, Y. (2017). EFFECT OF NITROGEN SUPPLY IN CULTURE MEDIA AND LIGHT INTENSITY ON PHOTOSYNTHESIS OF <i>Chlamydomonas reinhardtii</i>. Jurnal Bioteknologi &Amp; Biosains Indonesia (JBBI), 4(2), 64–69. https://doi.org/10.29122/jbbi.v4i2.15
Research Articles


Ball SG, Dirich L, Decq A, Marhat JC, Matagne R (1990) Psysiology of starch storage in the monocellular alga Chlamydomonas reinhardtii. Plant Sci 66:1-9

Chen M, Tang H, Ma H, Holland TC, Ng K, Sailey SO (2011) Effect of nutrients on growth and lipid accumulation in the green algae Dunaiella tertiolecta. Bioresour Technol 102:1649-1655

De Groot CC, Boogaard RVD, Marcelis LFM, Harbinson J, Lambers H (2003) Contrasting effects of N and P deprivation on the regulation of photosynthesis in tomato plants in relation to feedback limitation. J Exp Bot 54:1957-1967

Forde BG (2002) Local and long-range signaling pathway's regulating plant responses to nitrate. Annu Rev Plant Biol 53:203-224

Gargouri M, Bates PD, Park JJ, Kirchhoff H, Gang DR (2017) Functional photosystem I maintains proper energy balance during nitrogen depletion in Chlamydomonas reinhardtii, promoting triacylglycerol accumulation. Biotechnol Biofuels 10:89

Harris EH (1989) The Chlamydomonas Sourcebook: a Comprehensive Guide to Biology and Laboratory Use. Academic Press, San Diego

Johnson X, Alric J (2013) Central carbon metabolism and electron transport in Chlamydomonas reinhardtii: metabolic constraints for carbon partitioning between oil and starch. Eukaryot Cell 12:776-793

Juergens MT, Deshpande RR, Lucker BF, Park JJ, Wang H, Gargouri M, Kramer DM (2015) The regulation of photosynthetic structure and function during nitrogen deprivation in Chlamydomonas reinhardtii. Plant Physiol 167:558-573

Ikaran Z, Suárez-Alvarez S, Urreta I, Castañón S (2015) The effect of nitrogen limitation on the physiology and metabolism of Chlorella vulgaris var L3. Algal Res 10:134-144

Lardon L, Helias A, Sialve B, Steyer JP, Bernard O (2009) Life-cycle assessment of biodiesel production from microalgae. Environ Sci Technol 43: 6475-6481

Lee DY, Park JJ, Barupal DK, Fiehn O (2012) System response of metabolic networks in Chlamydomonas reinhardtii to total available ammonium.  Mol Cell Proteomics 11:973-988

Li Y, Han D, Sommerfeld M, Hu Q (2011) Photosynthetic carbon partitioning and lipid production in the oleaginous microalga Pseudochlorococcum sp. (Chlorophyceae) under nitrogen-limited conditions. Bioresour Technol 102:123-129

Ozawa S, Onishi T, Takahashi Y (2010) Identification and characterization of an assembly intermediate subcomplex of photosystem I in the green alga Chlamydomonas reinhardtii. J Biol Chem  285:20072–20079

Tan KWM, Lin H, Shen H, Lee YK (2016) Nitrogen-induced metabolic changes and molecular determinants of carbon allocation in Dunaliella tertiolecta. Scientific Reports, 6

Park JJ, Wang H, Gargouri M, Deshpande RR, Skepper JN, Holguin FO, Gang DR (2015) The response of Chlamydomonas reinhardtii to nitrogen deprivation: a systems biology analysis. Plant J 81:611-624

Wang ZT, Ulrich N, Joo S, Waffenschmidt S, Goodenough U (2009) Algal lipid bodies: stress induction, purification and biochemical characterization in wild type and starchless Chlamydomonas reinhardtii. Eukaryot Cell 8: 1856-1868

Most read articles by the same author(s)