AKTIVITAS <em>Stenotrophomonas rhizophila</em> DAN <em>Trichoderma</em> sp. DALAM MENGHAMBAT PERTUMBUHAN <em>Ganoderma boninense</em>
Main Article Content
Abstract
Activities of Stenotrophomonas rhizophila and Trichoderma sp. in Inhibiting the Growth of Ganoderma boninenseÂ
ABSTRACT
Basal stem rot (BSR) disease in oil palm (Elaeis guineensis Jacq.) due to infection of Ganoderma boninense. Various efforts to overcome BSR disease has been done, such as by utilizing endophytic microbes. The purpose of this research was to determine the activities of Stenotrophomonas rhizophila and Trichoderma sp inhibiting the growth of G. boninense. This research was divided into three stages, namely: stability test of S. rhizophila activity against G. boninense; activity of chitinase and cellulase enzymes produced by S. rhizophila; the effectiveness of S. rhizophila and Trichoderma sp. on G. boninense in a greenhouse. The parameters observed were plant height, leaves number, chlorophyll content, disease incidence and severity. The stability testing of S. rhizophila activity against G. boninense showed 53% of inhibition. Chitinase activity showed negative result. While cellulase index was about 0.46. The effectiveness test showed the significantly different results on plant height, leaves number and chlorophyll content.
Keywords: Chitinase, cellulase, Ganoderma boninense, Stenotrophomonas rhizophila, Trichoderma sp.
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ABSTRAK
Penyakit busuk pangkal batang (BPB) pada tanaman kelapa sawit (Elaeis guineensis Jacq.) muncul karena diinfeksi oleh Ganoderma boninense. Berbagai upaya penanggulangan penyakit BPB telah dilakukan, diantaranya dengan memanfaatkan mikroba endofit. Tujuan dari penelitian ini adalah untuk mengetahui aktivitas bakteri Stenotrophomonas rhizophila dan Trichoderma sp. dalam menghambat pertumbuhan G. boninense. Penelitian ini dibagi menjadi tiga tahapan, yaitu: pengujian stabilitas aktivitas S. rhizophila terhadap G. boninense; pengujian aktivitas enzim kitinase dan selulase yang dihasilkan oleh S. rhizophila; pengujian efektivitas S. rhizophila dan Trichoderma sp. terhadap G. boninense di rumah kaca. Parameter yang diamati pada pengujian efektivitas berupa tinggi tanaman, jumlah daun, jumlah klorofil, kejadian dan keparahan penyakit. Uji stabilitas aktivitas S. rhizophila terhadap G. boninense menunjukkan adanya penghambatan rata-rata sebesar 53%. Uji aktivitas enzim kitinase pada bakteri S. rhizophila menunjukkan hasil negatif. Sedangkan indeks enzim selulase pada bakteri S. rhizophila sebesar 0.46. Pada uji efektivitas tampak hasil yang berbeda nyata pada parameter tinggi tanaman, jumlah daun dan kandungan klorofil.
Kata Kunci: Kitinase, selulase, Ganoderma boninense, Stenotrophomonas rhizophila, Â Â Â Trichoderma sp.
Article Details
References
Abdullah F, Ilias GNM, Nelson M, Izzati NAMZ, Yusuf UK (2003) Disease assessment and the efficacy of Trichoderma as a biocontrol agent of basal stem rot of oil palms. Res Bul Sci Putra 11: 31-33
Alexander A, Dayou J, Chong KP (2015) Morphological changes of Ganoderma boninense mycelia after challenged by Tricoderma and Bacillus. AIP Conference Proceedings 1669: 020075
Anggarwulan E, Solichatun, Mudyantini W (2008) Karakter fisiologi kimpul (Xanthosoma sagittifolium (L.) Schott) pada variasi naungan dan ketersediaan air. Biodiversitas 9: 264-268. doi: 10.13057/biodiv/d090405
Dirjen Perkebunan (2015) Statistik Perkebunan Indonesia Komoditas Kelapa Sawit 2014-2016. Kementerian Pertanian
El-Katatny MH, Gudelj M, Robra KH, Elnaghy MA, Gübitz GM (2001). Characterization of a chitinase and an endo-β-1, 3-glucanase from Trichoderma harzianum Rifai T24 involved in control of the phytopathogen Sclerotium rolfsii. Appl Microbiol Biotechnol 56: 137-143. PMID: 11499921
Harris LK, Theriot JA (2016) Relative rates of surface and volume synthesis set bacterial cell size. Cell 165: 1479-1492. doi: 10.1016/j.cell.2016.05.045
Haryati T, Marbun PA, Purwadaria T (2010) Preservasi xilanase Bacillus pumilus PU4-2 dengan teknik imobilisasi pada pollard dan penambahan kation. JITV 15: 63-71
Musa H, Ali NBS (2017) Trichoderma spp consortium and its efficacy as biological control agent of Ganoderma disease of oil palm (Elaeis guineensis Jacquin). World Academy of Science, Engineering and Technology. Proceeding 19th International Conference on Forest Mycology and Plant Pathology. November 13-14, 2017, Tokyo
Naher L, Yusuf UK, Siddiquee S, Ferdous J, Rahman MA (2012) Effect of media on growth and antagonistic activity of selected Trichoderma strains against Ganoderma. Afr J Microbiol Res 6: 7449-7453. doi: 10.5897/AJMR12.1216
Naher L, Yusuf UK, Ismail A, Tan SG, Mondal MMA (2013) Ecological status of Ganoderma and basal stem rot disease of oil palms (Elaeis guineensis Jacq.). Aust J Crop Sci 7: 1723-1727
Naher L, Mokhtar SI, Sidek N (2015). Tricoderma harzianum T32 growth and antagonistic performance against Ganoderma boninense on different culture media. Pp 33-35. Proceeding 3rd International Conference on Biological, Chemical and Environmen-tal Sciences (BCES-2015). September 21-22, 2015, Kuala Lumpur Malaysia
Paterson RRM (2007) Ganoderma disease of oil palm: A white rot perspective necessary for integrated control. Crop Protect 26: 1369-1376. doi: 10.1016/ j.cropro.2006.11.009
Susanto A (2011) Ganoderma di perkebunan kelapa sawit dari waktu ke waktu. Simposium Nasional dan Lokakarya Ganoderma: Sebagai Patogen Penyakit Tanaman dan Bahan Baku Obat Tradisional. 2-3 November 2011, Bogor
Umrah, Anggraeni T, Esyanti RR, Aryantha INP (2009) Antagonisitas dan efektivitas Trichoderma sp. dalam menekan perkembangan Phytophthora palmivora pada buah kakao. Agroland 16: 9-16. doi: 10.22487/J.24077607.2009.v16.i1.211
Yaqub F, Shahzad S (2011) Efficacy and persistence of micobial antagonists against Sclerotium rolfsii under field conditions. Pak J Bot 43: 2627-2634
Ziedan EH, Elewa IS, Mostafa MH, Sahab AF (2011) Application of mycorrhizae for controlling root diseases of sesame. J Plant Protect Res 51: 355-361. doi: 10.2478/v10045-011-0058-0