THE REDUCTION OF Cr(VI) IN SOIL BY Microbacterium sp. STRAIN SpR3 IN VERMICOMPOST CARRIER

Main Article Content

Theodorus Olwyn Innation
Vincentia Irene Meitiniarti
Desti Christian Cahyaningrum

Abstract

Reduksi Cr(VI) pada Tanah Menggunakan Microbacterium sp. Strain SpR3 dengan Bahan Pembawa Vermikompos


Kromium hexavalen [Cr(VI)] adalah polutan yang berasal dari kegiatan industri. Microbacterium sp. strain SpR3 dapat digunakan sebagai agen bioremediasi Cr(VI). Kemampuan agen biologi untuk mereduksi Cr(VI) umumnya meningkat bila diinokulasi dalam media pembawa. Tujuan penelitian ini adalah untuk menganalisis kemampuan Microbacterium sp. strain SpR3 dalam mengurangi Cr(VI) serta membandingkannya dengan kondisi ketika diinokulasi dalam media pembawa vermikompos. Pengamatan dilakukan selama tujuh hari pada tiga perlakuan yang berbeda, yakni Microbacterium sp. strain SpR3 dengan dan tanpa media pembawa vermikompos yang diinokulasi pada tanah steril yang mengandung 50 ppm Cr(VI), dan tanah steril tanpa inokulasi bakteri yang mengandung 50 ppm Cr(VI). Variabel yang diamati adalah jumlah bakteri, konsentrasi, dan kecepatan reduksi Cr(VI) oleh bakteri tersebut di dalam tanah pada T0 (hari ke-0) dan T7 (hari ke-7). Hasil penelitian ini menyimpulkan bahwa vermikompos bisa digunakan sebagai media pembawa Microbacterium sp. strain SpR3 karena dapat meningkatkan jumlah bakteri sampai 2 × 1010 CFU g-1 di dalam tanah dan dapat mengurangi Cr(VI) dengan kecepatan 0,095 mg L-1 jam-1.


Hexavalent chromium [Cr(VI)] is a pollutant originated from industrial activities. Microbacterium sp. strain SpR3 can be used as Cr(VI) bioremediation agent. The ability of bioagent to reduce Cr(VI) usually improves when inoculated in a carrier. This research aimed to assess the ability of Microbacterium sp. strain SpR3 to reduce Cr(VI) in soil and compare its ability when inoculated in vermicompost carrier. Observations were carried out for seven days on three different treatments, namely Microbacterium sp. strain SpR3 with and without vermicompost inoculated in sterile soils containing 50 ppm Cr(VI), and sterile soils containing 50 ppm Cr(VI) without bacterial inoculation. The observed variables were the number of bacteria, the concentration of Cr(VI) and the rate of Cr(VI) reduction by these bacteria in the soil at T0 (day 0) and T7 (day 7). It was concluded that vermicompost could be used as a carrier of Microbacterium sp. strain SpR3 as it could increase the number of the bacteria to 2 × 1010 CFU g-1 in soil and could reduce Cr(VI) at the rate of 0.095 mg L-1 h-1.

Article Details

How to Cite
Innation, T. O., Meitiniarti, V. I., & Cahyaningrum, D. C. (2021). THE REDUCTION OF Cr(VI) IN SOIL BY Microbacterium sp. STRAIN SpR3 IN VERMICOMPOST CARRIER. Jurnal Bioteknologi &Amp; Biosains Indonesia (JBBI), 8(1), 33–41. https://doi.org/10.29122/jbbi.v8i1.4160
Section
Research Articles
Author Biographies

Theodorus Olwyn Innation, Universitas Kristen Satya Wacana

Fakultas Biologi

Vincentia Irene Meitiniarti, UNIVERSITAS KRISTEN SATYA WACANA

FAKULTAS BIOLOGI

Desti Christian Cahyaningrum, Universitas Kristen Satya Wacana

Fakultas Biologi

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