KARAKTERISASI ISOLAT BAKTERI FIBRINOLITIK WU 021055* ASAL PERAIRAN PANTAI PAPUMA, JEMBER
Article Sidebar
Main Article Content
Abstract
A blood clot (thrombus) in a blood stream is formed due to a circulatory system imbalance in the hemostasis which results in plug of blood vessels. The suppliy of nutrients and oxygen to the tissues is inhibited (ischemia) by the accumulation of thrombus and embolus in the blood vessel. This prosses is the main cause for further atherotrombotic diseases such as myocardial infraction and cerebral infraction. This disease could be overcome by thrombolytic therapy by using fibrinolytic protease enzyme. Fibrinolytic activity of protease enzymes have been studied from various species of bacteria. Bacterial isolate of WU 021055* obtained from Papuma coastal waters has demonstrated fibrinolytic activity. This research was aimed to identify the bacterial isolate through morphological characterization (colony and cell morphology), physiological characterization (indole test, carbohydrates fermentation test (glucose, lactose, sucrose and fructose), catalase test, starch hydrolysis test, and the pH effect test), and molecular identification using 16S rRNA. Based on those characterizations, the bacterial isolate of WU 021055* shows a high similarity to Bacillus aerius.
Keywords: Atherotrombosis, fibrinolytic, identification, characterization, bacteria
Â
ABSTRAK
Bekuan darah (trombus) dalam peredaran darah terbentuk akibat ketidakseimbangan sistem sirkulasi dalam hemostasis yang menyebabkan penyumbatan pembuluh darah. Akumulasi trombus dan embolus pada pembuluh darah mengakibatkan suplai nutrisi dan oksigen ke jaringan terhambat (iskemia) dan bahkan kematian jaringan (infark). Pembentukan ini merupakan etiologi dari penyakit aterotrombosis seperti infark miokard dan infark serebral. Penyakit akibat trombosis ini dapat diatasi dengan terapi trombolitik dengan enzim protease fibrinolitik. Aktivitas enzim protease fibrinolitik telah diteliti dari berbagai spesies bakteri. Isolat bakteri WU 021055* asal perairan pantai papuma tampak memiliki aktivitas fibrinolitik. Pada penelitian ini dilakukan identifikasi isolat bakteri melalui karakterisasi morfologi (morfologi koloni dan sel), karakterisasi fisiologis (uji indol, uji fermentasi karbohidrat (glukosa, laktosa, sukrosa dan fruktosa), uji katalase, uji hidrolisis pati, dan uji pengaruh pH), dan identifikasi secara molekuler menggunakan 16S rRNA. Berdasarkan karakterisasi morfologi, fisiologi, dan marker 16S rRNA, isolat bakteri WU 021055* menunjukkan kemiripan yang tinggi dengan Bacillus aerius.
Kata kunci: Aterotrombosis, fibrinolitik, identifikasi, karakterisasi, bakteri
Article Details
References
Cappucino JG, Sherman N (1987) Microbiology, A Laboratory Manual. California. Menko Park The Benjamin/ Cummins Publishing Company, Menlo Park, California
Chasanah E (2009) Marine biodiscovery research In Indonesia: challenges and rewards. J Coastal Dev 12:1-12
Claus D, Berkeley CW (1984) Endospore-Forming Rods and Cocci. Dalam: NR Krieg & JG Jolt (ed.), Bergeys Manual of Determinative Bacteriology. Williams and Wilkins, Baltimore: 529–551
Cowan ST (1974) Manual for the Identification of Medical Bacteria. Cambridge University Press, London
Drancourt M, Bollet C, Carlioz A, Martelin R, Gayral JP, Raoult D (2000) 16S ribosomal DNA sequence analysis of a large collection of environmental and clinical unidentifiable bacterial isolates. J Clin Microbiol 38:3623-3630
Escobar CE, Harmaening DM, Simmons VL, Smith, Moore KM, Wyrick-Glatzel J (2002) Introduction to Hemostasis. Dalam: DM Harmening (ed.), Clinical Hematology and Fundamentals of Hemostasis. (Edisi Keempat) FA Davis, Philadelphia
Jawetz M, Adelberg (2008) Mikrobiologi Kedokteran. Alih Bahasa oleh Huriwati Hartanto. Penerbit Buku Kedokteran ECG, Jakarta
Lane DJ, Pace B, Olsen GJ, Stahl DA, Sogin ML, Pace NR (1985) Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. Proc Natl Acad Sci USA 82:6955–6959
Sravankumar G, Durgaprasad YVK, Ramana T, Hasasreeramulu, Devi CSLV (2010) Isolation and identification of bacteria from marine biofilm on the artificial plat forms (iron panels) from Visakhapatham coast, India. Indian J Mar Sci 43:955-959
Kumada K, Onga T, Hoshino H (1994) The effect of natto possessing a high fibrinolytic activity in human plasma. Igaku to Seibutsugaku 128:117-119
Oswald N (2007) Quick reference: Determining DNA Concentration and Purity. [online] http://bitesizebio.com/13501/ dna-concentration-purity/ diakses 17 Desember 2015
Prasad S, Kashyap RS, Deopujari JY, Purohit HJ, Taori GM, Daginawala HF (2007) Effect of Fagonia Arabica (Dhamasa) on in vitro Thrombolysis. BMC Complem Altern Med 7:36
Setiawan A (2013) Skrining Agen Fibrinolitik Isolat Bakteri dari Perairan pantai papuma kabupaten jember. Skripsi fakultas kesehatan masyarakat Universitas Jember
Sajuthi, Suparto, Yanti, Praira (2010) Purifikasi dan Pencirian Enzim Protease Fibrinolitik dari Ekstrak Jamur Merang. Makara Sains 14:145-150
World Health Organization (2008) The World Health Report 2008: Primary Health Care Now More Than Ever. Geneva: WHO Library Cataloguing-in-Publication
Xin ZXYHX, Baocheng Z (2012) Enzymological Properties and Thrombolysis Effect in Vitro of Fibrinolytic Enzyme S-7FE-1. J Chin Ins Food Sci Tech 10:013. Jurnal online http:// en.cnki.com.cn/Article_en/CJFDTOTAL-ZGSP201210013.htm diakses 12 Juli 2015