Main Article Content

Indri Handayani
Farida Rosana Mira
Zhafira Amila Haqqa
Nia Asiani
Abdul Wahid
Mahmud Sugianto
R Bambang Sukmadi
Bedah Rupaedah



Centrosema pubescens is a legume plant that is commonly used as animal feed, cover crop, and one of the plants used to reclaim critical land. This study was conducted to increase the germination of C. pubescens seeds by coating the seeds using a coating material enriched with a consortium of microbes consisting of nitrogen-fixing bacteria, phytohormones-producing bacteria, phosphate-solubilizing bacteria, and arbuscular mycorrhizal fungi. Germination test on control, seed coating, and microbial seed coating was carried out using the top of the sand method for 28 days. Observational data were tested using ANOVA statistics and LSD tests. Results of the research showed the moisture content of the seeds was 12.45%, the purity of the seeds was 95.11% and the weight of 1000 seeds was 23.74 g. The germination test denoted that the number of normal germinated seeds in seed coating and microbial seed coating was significantly different from the control. In addition, dead seeds in microbial seed coating treatment had the lowest value and were significantly different from other treatments. These results indicate that microbial seed coating can increase seed germination and considerably reduce seed death due to seed-borne pathogens.



Centrosema pubescens adalah tanaman kacang-kacangan yang dimanfaatkan sebagai pakan ternak, penutup tanah dan salah satu tanaman yang digunakan di lahan kritis. Penelitian ini dilakukan untuk meningkatkan perkecambahan benih C. pubescens dengan melakukan pelapisan benih yang diperkaya dengan bakteri penambat nitrogen, bakteri penghasil fitohormon, bakteri pelarut fosfat dan cendawan mikoriza arbuskular.  Uji Daya berkecambah dilakukan pada benih kontrol, benih berlapis dan benih berlapis mikroba menggunakan metode penanaman benih di atas pasir selama 28 hari. Data pengamatan dianalisis menggunakan ANOVA dan uji lanjut LSD. Hasil penelitian menunjukkan kadar air benih C. pubescens 12,45%, kemurnian benih 95,11% dan bobot 1000 benih 23,74 g. Daya berkecambah benih menunjukkan perlakuan benih berlapis mikroba berbeda signifikan dengan kontrol. Persentase benih mati pada perlakuan benih berlapis mikroba memiliki nilai paling kecil dan berbeda nyata terhadap perlakuan lainnya. Hal ini mengindikasikan bahwa pelapisan benih menggunakan mikroba dapat meningkatkan persentase perkecambahan benih dan mengurangi jumlah benih mati akibat patogen bawaan benih.

Article Details

How to Cite
Handayani, I., Mira, F. R., Haqqa, Z. A., Asiani, N., Wahid, A., Sugianto, M., Sukmadi, R. B., & Rupaedah, B. (2022). EFFECTS OF SEED COATING USING PLANT-BENEFICIAL MICROBES ON THE GERMINATION OF Centrosema pubescens SEEDS. Jurnal Bioteknologi &Amp; Biosains Indonesia (JBBI), 9(2), 237–246. https://doi.org/10.29122/jbbi.v9i2.5541
Research Articles


Alghamdi SA (2019) Influence of mycorrhizal fungi on seed germination and growth in terrestrial and epiphytic orchids. Saudi J Biol Sci 26:495–502. doi: 10.1016/j.sjbs.2017.10.021

Amza J (2018) Seed borne fungi; food spoilage, negative impact and their management: A review. Food Sci Qual Manag 81:70-79

Araújo WL, Creason AL, Mano ET, Camargo-Neves AA, Minami SN, Chang JH, Loper JE (2016) Genome sequencing and transposon mutagenesis of Burkholderia seminalis TC3.4.2R3 identify genes contributing to suppression of orchid necrosis caused by B. gladioli. Mol Plant-Microbe Interact 29:435–446. doi: 10.1094/MPMI-02-16-0047-R

Di Benedetto NA, Corbo MR, Campaniello D, Cataldi MP, Bevilacqua A, Sinigaglia M, Flagella Z (2017) The role of plant growth promoting bacteria in improving nitrogen use efficiency for sustainable crop production: A focus on wheat. AIMS Microbiol 3:413–434. doi: 10.3934/microbiol.2017.3.413

dos Santos AC, Kandasamy S, Rigobelo EC (2020) Bacillus cereus, Lactobacillus acidophilus and Succinovibrio dextrinosolvens promoting the growth of maize and soybean plants. Afr J Microbiol Res 14:189–197. doi: 10.5897/ajmr2020.9322

Egamberdieva D, Wirth SJ, Alqarawi AA, Abd-Allah EF, Hashem A (2017) Phytohormones and beneficial microbes: Essential components for plants to balance stress and fitness. Front Microbiol 8:2104. doi: 10.3389/fmicb.2017.02104

Etesami H, Maheshwari DK (2018) Use of plant growth promoting rhizobacteria (PGPRs) with multiple plant growth promoting traits in stress agriculture: Action mechanisms and future prospects. Ecotoxicol Environ Saf 156:225–246. doi: 10.1016/j.ecoenv.2018.03.013

ISTA (2014) International rules for seed testing. International Seed Testing Association, Bassersdorf

Kumar V, Sridhar K (2015) Seed standards and seed testing protocols for forage legume seeds. Int Grassl Congr Proc. https://uknowledge.uky.edu/igc/23/2-7-1/3. Accessed 2 June 2022

Lal M, Kumar A, Chaudhary S, Singh RK, Sharma S, Kumar M (2022) Antagonistic and growth enhancement activities of native Pseudomonas spp. against soil and tuber-borne diseases of potato (Solanum tuberosum L.). Egypt J Biol Pest Control 32:22. doi: 10.1186/s41938-022-00522-w

Lami MJ, Adler C, Caram-Di Santo MC, Zenoff AM, de Cristóbal RE, Espinosa-Urgel M, Vincent PA (2020) Pseudomonas stutzeri MJL19, a rhizosphere-colonizing bacterium that promotes plant growth under saline stress. J Appl Microbiol 129:1321–1336. doi: 10.1111/jam.14692

Liu S, Lv D, Lu C, Xiao Y, Wang S, Zhou W, Niu J, Wang Z (2022) Tulasnella can contribute to breakthrough in germination of the medicinal orchid Bletilla striata via producing plant hormones. Rhizosphere 22:100527. doi: 10.1016/j.rhisph.2022.100527

Lusembo P, Sabiiti EN, Mugerwa JS (1995) Influence of seed size on and seeling characteristics of Centrosema pubescens. Afr Crop Sci J 3:547-551. doi: 10.4314/acsj.v3i4.54511

Ma Y (2019) Seed coating with beneficial microorganisms for precision agriculture. Biotechnol Adv 37:107423. doi: 10.1016/j.biotechadv.2019.107423

Ma Y, Látr A, Rocha I, Freitas H, Vosátka M, Oliveira RS (2019) Delivery of inoculum of Rhizophagus irregularis via seed coating in combination with Pseudomonas libanensis for cowpea production. Agronomy 9:33. doi: 10.3390/agronomy9010033

Mancini V, Romanazzi G (2014) Seed treatments to control seedborne fungal pathogens of vegetable crops. Pest Manag Sci 70:860–868. doi: 10.1002/ps.3693

McCormack JH (2004) Seed processing and storage: Principles and practices of seed harvesting, processing, and storage: an organic seed production manual for seed growers in the Mid-Atlantic and Southern U.S. www.savingourseeds.org . Accessed 27 May 2022

Moradtalab N, Ahmed A, Geistlinger J, Walker F, Höglinger B, Ludewig U, Neumann G (2020) Synergisms of microbial consortia, N forms, and micronutrients alleviate oxidative damage and stimulate hormonal cold stress adaptations in maize. Front Plant Sci 11:396. doi: 10.3389/fpls.2020.00396

Paradelo R, Basanta R, Barral MT (2019) Water-holding capacity and plant growth in compost-based substrates modified with polyacrylamide, guar gum or bentonite. Sci Hortic (Amsterdam) 243:344–349. doi: 10.1016/j.scienta.2018.08.046

Paravar A, Piri R, Balouchi H, Ma Y (2022) Microbial seed coating: an attractive tool for sustainable agriculture. Authorea May 17, 2022. doi: 10.22541/au.165276913.31013497/v1

Pedrini S, Merritt DJ, Stevens J, Dixon K (2017) Seed coating: Science or marketing spin? Trends Plant Sci. 22:106–116. doi: 10.1016/j.tplants.2016.11.002

Rahmawati, Aqil M (2020) The effect of temperature and humidity of storage on maize seed quality. IOP Conf Ser: Earth Environ Sci 484:012116. doi: 10.1088/1755-1315/484/1/012116

Rocha I, Ma Y, Souza-Alonso P, Vosátka M, Freitas H, Oliveira RS (2019) Seed coating: A tool for delivering beneficial microbes to agricultural crops. Front Plant Sci 10:1357. doi: 10.3389/fpls.2019.01357

Rusdy M (2015) Enhancing germination in seeds of Centrosema pubescens. Int J Sci Res Publications 5. Corpus ID: 150367241

Sarjono A, Guntoro D, Supijatno (2019) Perbandingan Arachis pintoi dengan tanaman kacang-kacangan penutup tanah lain dalam menekan laju erosi pada lahan kelapa sawit berbukit. J Agron Indones 47:90–96. doi: 10.24831/jai.v47i1.22891

Siddique AB, Wright D (2003) Effect of different drying time and temperature on moisture percentage and seed quality (viability and vigour) of pea seeds (Pisum sativum L.). Asian J Plant Sci 2:978–982. doi: 10.3923/ajps.2003.978.982

Sudrajat DJ, Nurhasybi, Bramasto Y (2015) Standar Pengujian dan Mutu Benih Tanaman Hutan. Forda Press, Bogor

Susilowati DN, Riyanti EI, Setyowati M, Mulya K (2018) Indole-3-acetic acid producing bacteria and its application on the growth of rice. AIP Conf Proc 2002:020016. doi: 10.1063/1.5050112

Tambunan RP, Sukoso, Syekhfani, Priatmadi BJ (2017) Use of vegetation cover for erosion control physical characteristics of soil improvement in post coal mining in South Kalimantan. IOSR J Environ Sci 11:11–16. doi: 10.9790/2402-1110011116

Tang A, Haruna AO, Majid NMA, Jalloh MB (2020) Potential PGPR properties of cellulolytic, nitrogen-fixing, phosphate-solubilizing bacteria in rehabilitated tropical forest soil. Microorganisms 8:442. doi: 10.3390/microorganisms8030442

Weller DM (2007) Pseudomonas biocontrol agents of soilborne pathogens: Looking back over 30 years. Phytopathology 97:250–256. doi: 10.1094/PHYTO-97-2-0250

Yilmaz E, Sonmez I, Demir H (2014) Effects of zeolite on seedling quality and nutrient contents of cucumber plant (Cucumis sativus L. cv. Mostar F1) grown in different mixtures of growing media. Commun Soil Sci Plant Anal 45:2767–2777. doi: 10.1080/00103624.2014.950425

Zhang F, Zou YN, Wu QS, Kuca K (2020) Arbuscular mycorrhizas modulate root polyamine metabolism to enhance drought tolerance of trifoliate orange. Environ Exp Bot 171:103926. doi: 10.1016/j.envexpbot.2019.103926

Most read articles by the same author(s)