MUTATION OF LIPASE-PRODUCING BACTERIAL ISOLATE FROM PALM OIL EFFLUENT FOR FAT HYDROLYSIS ON POME
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Abstract
Lipase digunakan untuk menghidrolisis minyak atau lemak yang tersisa dalam limbah cair pabrik kelapa sawit (POME), yang digunakan sebagai bahan baku dalam pembentukan biogas. Isolat bakteri (Bacillus velezensis) yang menunjukkan aktivitas lipase, sebelumnya diperoleh dari Malimping, Pandeglang, Banten, Indonesia, mengalami perlakuan berurutan yaitu iradiasi gamma (1, 2, dan 3 kGy), mutagenesis EMS (ethyl methane sulfonate) (0,5% v/v), dan NTG (N-methyl N-nitrosoguanidine) (1 mg mL-1). Setelah setiap mutasi, aktivitas lipase dari koloni mutan diukur dan dibandingkan dengan strain tipe liar. Hasil penelitian menunjukkan bahwa semua koloni B. velezensis yang bermutasi menunjukkan nilai aktivitas lipase (7,78 ± 0,80 – 9,05 ± 0,23 U mL-1) yang tidak berbeda signifikan dengan isolat tipe liar (8,31 ± 0,01 U mL-1), menunjukkan ketahanan bakteri yang kuat terhadap perlakuan mutagenik. Preparasi lipase kasar secara efektif menghidrolisis POME, mengakibatkan penurunan nilai COD (dari 131.450 ppm menjadi 88.450 ppm) dan kandungan O&G (dari 41.400 mg L-1 menjadi 5.770 mg L-1) dalam jangka waktu 72 jam.
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References
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