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Coconut water and rotten banana fruits are commonly found in traditional markets asÂ organic wastes. One way to overcome the problems caused by these organic wastesÂ is to convert these unuseful matter into an important and economically useful matterÂ by using them as components of tissue culture media. One important commodity thatÂ is usually propagated by tissue culture is Phalaenopsis orchid type 229 (PhalaenopsisÂ amabilis). Therefore, it would be more benefit to substitute the expensive chemicalsÂ with organic wastes such as coconut water and banana puree.In this experiment, addition of coconut water and banana pure to the minimum mediaÂ containing commercial fertilizer red Polyhyponex, sucrose and commercial agar did notÂ show any inhibition of Phalaenopsis orchid plantlet growth. This probably caused byÂ sufficient macro and micro nutrients provided by those organic matter and Polyhyponex
fertilizer. Moreover, addition of 100 mL/L of coconut water and 100 mg/L banana pureeÂ gave the optimum leaf and adventitious shoot formation. On the other hand, additionÂ of 150 mL/L coconut water gave the optimum height and root formation. In this case,growing Phalaenopsis orchid plantlet should be done in 2 subculture period. The firstÂ subculture is to obtain maximum amount of leaf and shhot formation while the secondÂ subculture is to obtain optimum height and root formation.
Key words : Waste, coconut water, banana puree, tissue culture, Phalaenopsis orchi
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