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The aim of the research was to know the capability of actinomycetes isolate fromÂ Bangka Belitung, which stored at Microbiology-LIPI Culture Collection, in producingÂ chitinase enzyme. This isolate which could produce chitinolitic enzyme, signed byÂ clear zone at medium contain 1% chitine. The chitinase activity of the isolate whichÂ incubated for 1-7 days in the room temperature was analyzed by spectrophotometer inÂ Î» 584 nm. The result of this experiment was highest chitinase activities with incubatedÂ for 3 days, were 1.66 . 10-2 U/ml. Maximum chitinase activities was found at 1% starchÂ soluble substrate 2.83 . 10-2, pH 8.0 and at 50Â°C condition were 9.3 . 10-2 and 12.98 .10-2 U/ml respectively.
Key words : chitinase, clear zone, spectrophotometer
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