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The activity of phosphate solubilizing enzyme and the effectivity of microbe fromÂ Wamena for support agriculture of environmental friendliness at marginal area.Â Phosphate dissolution of enzyme by microbial effective in compost plus from WamenaÂ forest was stored in the freezer for 4 years, have been conducted in EcofisiologyÂ laboratory,Microbiology devision, Research Center for Biology, Indonesian Institute ofÂ Science. This research was conducted to know the microbial affectivity and the activityÂ of phosphate solubilizing enzyme of 20 microorganisms in inoculants stored 4 years inÂ refrigerated room and to support soil fertility and biofertilizer agent in organic farmingÂ system. This research used plate count methode for counting the amount of microbialÂ population, Scinner method was used toanalizer enzyme of alkaline and acid PME-ase,and statistical analysis use Duncan methode. The storage of 20 inoculants invected 20
microorganisms for 4 years in refrigerated room showed that the microbial activity stillÂ high with the amount of mean population 107 and the activity of phosphate solubilizingÂ enzyme with mean diameter of clear zone (holozone) 1 cm â€“ 2 cm. The effectivity and theÂ highest activity of phosphate solubilizing enzyme were : Azotobacter indicus (A), BacillusÂ panthothenticus (D), Bacillus megaterium (M), Bacillus thuringiensis (O), Ceratia sp. (R),Chromobacterium lividum (G), Chromobacterium violaceum (S), Flavobacterium breve
(T), Klebsiella aerogenes (H), Pseudomonas fluorescent (J), Rhizobium legurxinosariumÂ (L), and the lowest were: Streptomyces sp. (I) .
Key word: Alkaline and acid PMEase, Phosphate solubilizing bacteria,Biofertolizer
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