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Picrasma javanica is one of the medicinal plants that has an anti malaria potency,it is belonging into Simaroebaceae family. The aim of this research is to know the effect of GA3 on broken dormancy of P. Javanica seed after storaged in the variousÂ temperature to enhance and increased seed germination. The experiment was carriedÂ out in the Macropropagation Laboratory, Cibinong Science Center, Research Center forÂ Biology,LIPI, using a Complete Randomized Design with two factors in three replication.The first factor is condition of storage i.e room (28 Â± 10 C, RH 80%), incubator (200 C,RH 90%), incubator ( 50 C, RH 95%) dan deep freezer (- 200 C, RH ). The second factorÂ is long of storage i.e. 1,2,3,4,5 and 5 months. GA3 100 ppm was used for broken seed
dormancy. The result showed that there was no interaction between temperature andÂ long of storage. GA3 solution can be broken on seed dormancy before and after storageÂ in the various temperature excepted in deep freezer ( â€“ 200 C, RH 65%), there are noÂ seed germinate since 1 month storage. P. javanica seed that storage in incubator 50 CÂ is the best method and after 5 months the germination percentage is still high (73,3%)Â with GA3 but only 18,33% without GA3. On the other treatments the germinationÂ percentage is less than 50%. The highest germination value (14,1) that was recordedÂ in the seeds that storage in incubator (200 C, RH. 95%).
Key words: GA3, dormancy, temperature, long of storage, seed , P. javanica
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