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use of chemical pesticides has caused serious environmental problems and thus the demand
for safer pesticides is increasing. One alternative is microbial pesticides that suppress fungal
and bacterial of plant pathogens. Bacillus subtilis has been known as producer of lipopeptide
antibiotics, like iturin A, plipastatin and surfactin. In this study, iturin A as an antifungal of plan
pathogens was produced on varieties of soybean meal concentration; i.e. 8%, 10%, 12%, and
15% using B.subtilis RB14-CS.
The result indicates that 8% soybean meal concentration produced the highest of iturin A
production ( 2469 mg L-1) compared to the others. Beside the effect of nitrogen source (
soybean), the influence of 20 kinds of L-amino acids on an enhancement of iturin A productivity
were observed. The examined L-amino acids were L-ala, L-arg, L-asn, L-asp, L-cys, L-glu, L-gln,
L-gly, L-his, L-ile, L-leu, L-lys, L-met, L-pro, L-phe, L-ser, L-thr, L-tyr, L-trp and L-val. The results
show that no one of them could improve the iturin A productivity on soybean meal medium.
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