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concentration of BOD using autotrophic bacteria were conducted in batch
suspension experiment to investigate nitrate concentration level which can be
treated by the bacteria. The autotrophic bacteria were enriched by acclimating
activated sludge with inorganic substrates. The experimental result shows that
denitrification reaction with initial nitrate concentration in synthetic wastewater from 200 to 850 mg/l proceeded according to the one-order reaction. The denitrification rate increased with increasing the initial nitrate concentration. Sulfate was detected as the by product of the denitrification reaction. The sulfate produced for 1 mmol of nitrate decreased was 1.09 mmol.
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