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toxicity test of waste, soil, and water. In this experiment Lemna sp. were
exposed to waste water collected from tanneries in Garut, West Java.
The experiment system includes liquid medium (macro and micro salts of
Murashige and Skoog), to which a series of concentrations of the pre-
filter-sterilized tannery waste water were added aseptically. Aseptic grown
Lemna sp. were placed on the medium in a density of 10 fronds per
medium container. The system was then incubated for 7 days under
continuous daylight fluorescent lamps and the number of fronds, fresh
weight and chlorophyll content of the plants were determined. The results
show that EC50 of the tannery waste water is 0.66% of waste water. The
LOEC value is 0.1% and the NOEC value could not be determined but
should below 0.1%. This experiment suggests that tannery waste water is
dangerous to aquatic plants if it was discharged to public water body
without appropriate pre-treatment.
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