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and a commercial detergent following method described by OECD. In the
test, 10 fronds of Lemna sp. were grown in a modified MS medium
containing the surfactants and detergent in the concentration of 0 ppm to
79 ppm and incubated for 7 days under continuous fluorescence lamps.
The EC50 values of the detergent and surfactants vary depending on the
compounds and the testing parameter. For LAS and ABS, the value of
EC50 from number of fronds is higher than EC50 from the total chlorophyll
content, i.e. 14.83 and 14.36 mg/l for LAS and 11.75 and 7.56 mg/l for
ABS, respectively. However, the total chlorophyll content is not sensitive
for commercial detergent “A” and results in a higher EC50 value of 31.53
mg/l. LOEC values for LAS and ABS is 5 mg/l respectively, and is 25 mg/
for detergent ”A”.
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