For the Comfort and Safety of Testing Personnel and the Safety for the Analysis Process and for the Environment from Exposure to SARS-CoV-2 Virus Contamination


  • Andhy Muhammad Fathoni Laboratory for Thermodynamics, Engines and Propulsion Technology, BPPT
  • Himawan Sutriyanto Laboratory for Thermodynamics, Engines and Propulsion Technology, BPPT
  • Achmad Maswan Laboratory for Thermodynamics, Engines and Propulsion Technology, BPPT
  • Kanon Prabandaru Sumarah Laboratory for Thermodynamics, Engines and Propulsion Technology, BPPT


Tracing, tracking and testing are keys in fighting COVID-19 pandemic. At the start of the pandemic in Indonesia, the capacity to test with RT-PCR methods as the golden standard was still quite low. BPPT, through TFRIC-19, developed MBSL-2 to increase the capacity of Covid-19 PCR Test. This Mobile Bio-Safety Level-2 (MBSL-2) is equipped with HVAC system which has the main function of providing comfort and safety for testing personnel as well as safety for the testing process. This MBSL-2 also provides safety for the environment in which MBSL-2 is placed from contamination of the SARS-CoV-2 virus which may be released during the PCR analysis process. There are several variants of the Heating, Ventilating and Air Conditioning (HVAC) MBSL-2 that have been built and operated. The requirement and the design result for MBSL-2 has been discussed in this paper. This HVAC system has to be able to cover cooling load of 2459 W. This MBSL-2 HVAC system has been designed to complement the RT-PCR testing facility that is safe for the personnel, environment and testing result from contamination. This MBSL-2 has been proved to be safe in the operation during this pandemic.

Keywords : PCR Test; HVAC; COVID-19; MBSL-2, BSL-2


D. Wang, B. Hu, C. Hu, et al. “Clinical characteristics of 138 hospitalized patients with 2019 Novel Coronavirus–infected pneumonia in Wuhan, China”, JAMA, 2020, pp. 1061-1069. DOI:10.1001/jama.2020.1585.

R. Hidayat, N. Aini, A.F.N. Ilmi, F. Azzahroh, and A. Giantini, “Test, trace, and treatment strategy to control COVID-19 infection among hospital staff in a COVID-19 referral hospital in Indonesia”, Acta medica Indonesiana-the Indonesian Journal of Internal Medicine, Vol. 52, 2020, pp.206-213.

WHO, “WHO Coronavirus Disease (COVID-19) dashboard”, 2020, Available from:

Scottish Government, “Covid-19 – Test, trace, isolate, support: A public health approach to maintaining low levels of community transmission of COVID-19 in Scotland”, 2020.

A. J. Kucharski, P. Klepac, A.J.K. Conlan, et al., “Effectiveness of isolation, testing, contact tracing, and physical distancing on reducing transmission of SARS-CoV-2 in different settings: a mathematical modelling study”, Lancet Infectious Disease, 2020.

National Institute of Health (NIH), “Technical resources-design requirements manual”, Office of Research Facilities, 2020.

Stanford University, “General: ventilation consideration”, cited: March 2021, Available from:

J. DeLaurentis, K. Biopharm, “Designing HVAC systems for biosafety level requirements”, cited: March 2021, Available from:

ASHRAE, I. B. D. McIntosh, C. B. Dorgan, and C. E. Dorgan, “Ashrae laboratory design guide – Planning and operation of laboratory HVAC systems”, American society of heating, refrigerating and air conditioning engineers (ASHRAE), 2002.

US Food and Drug Administration, “Ora Lab Manual Volume II”, Available from:

Siemens Industry, “Laboratory ventilation codes and standards”, 2013. Available from:

ANSI, “Laboratory ventilation (ANSI / AIHA Z9.5 –2012)”, The American society of safety engineers (ASSE), 2012.

Litbang PU, “Tata cara perancangan sistem ventilasi dan pengkondisian udara pada bangunan gedung”, Standard Nasional Indonesia (SNI), 2001.